PMID- 11012761
OWN - NLM
STAT- MEDLINE
DCOM- 20001016
LR  - 20190513
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 101
IP  - 1
DP  - 2000 Sep
TI  - Immunoglobulin E antibodies of atopic individuals exhibit a broad usage of 
      VH-gene families.
PG  - 112-9
AB  - The term 'atopy' describes the genetically determined tendency to mount 
      immunoglobulin E (IgE) antibody responses against per se harmless antigens 
      (allergens). In this study we investigated the usage of VH families in the 
      formation of IgE antibodies in 10 patients suffering from mucosal and/or skin 
      manifestations of atopy. IgE antibody reactivities to exogenous allergen sources 
      as well as to autoallergens were determined and, by immunoabsorption, it was 
      demonstrated that allergen-specific IgE accounted for most of the total serum IgE 
      levels in these patients. Using primers with specificity for the VH1-6 gene 
      families and a primer specific for the first constant region of human IgE, cDNAs 
      coding for IgE heavy chain fragments were amplified using the reverse 
      transcription-polymerase chain reaction (RT-PCR) from peripheral blood 
      lymphocytes of the 10 atopic individuals. Hybridization of the heavy 
      chain-encoding cDNAs with an IgE-specific internal oligonucleotide probe revealed 
      a broad usage of all VH-gene families in the atopic individuals. The spectrum of 
      VH families used in a given atopic individual was neither associated with the 
      type or severity of clinical symptoms nor with the number of allergens 
      recognized. The fact that allergen-specific IgE antibodies in atopic individuals 
      originate from a broad variety of B cells thus reflects the activation of 
      multiple B-cell clones during allergen sensitization. This finding should be 
      borne in mind if therapeutic strategies for Type I allergy are considered that 
      aim at a clonal elimination of allergen-specific B cells.
FAU - Eibensteiner, P
AU  - Eibensteiner P
AD  - Department of Pathophysiology, Institute of Medical and Chemical Laboratory 
      Diagnostics, University of Vienna, Vienna General Hospital, Austria.
FAU - Spitzauer, S
AU  - Spitzauer S
FAU - Steinberger, P
AU  - Steinberger P
FAU - Kraft, D
AU  - Kraft D
FAU - Valenta, R
AU  - Valenta R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Allergens)
RN  - 0 (DNA, Complementary)
RN  - 0 (Epitopes)
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Immunoglobulin Variable Region)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Allergens/immunology
MH  - DNA, Complementary/genetics
MH  - Epitopes
MH  - Female
MH  - *Genes, Immunoglobulin
MH  - Humans
MH  - Hypersensitivity, Immediate/*genetics/immunology
MH  - Immunoglobulin E/blood/*genetics
MH  - Immunoglobulin Heavy Chains/*genetics
MH  - Immunoglobulin Variable Region/*genetics
MH  - Male
MH  - Middle Aged
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC2327057
EDAT- 2000/09/30 11:00
MHDA- 2000/10/21 11:01
PMCR- 2001/09/01
CRDT- 2000/09/30 11:00
PHST- 2000/09/30 11:00 [pubmed]
PHST- 2000/10/21 11:01 [medline]
PHST- 2000/09/30 11:00 [entrez]
PHST- 2001/09/01 00:00 [pmc-release]
AID - imm078 [pii]
AID - 10.1046/j.1365-2567.2000.00078.x [doi]
PST - ppublish
SO  - Immunology. 2000 Sep;101(1):112-9. doi: 10.1046/j.1365-2567.2000.00078.x.