PMID- 11014616
OWN - NLM
STAT- MEDLINE
DCOM- 20010118
LR  - 20191104
IS  - 1138-7548 (Print)
IS  - 1138-7548 (Linking)
VI  - 56
IP  - 2
DP  - 2000 Jun
TI  - Growth hormone response to long-term GH-RH administration in lambs.
PG  - 107-15
AB  - The pattern of long-term GHRH administration capable of stimulating GH release 
      without depleting pituitary GH content has been investigated using two 
      experimental approaches. In experiment 1, recently weaned male lambs were treated 
      for 3 weeks as follows: Group A) control; B) subcutaneous (sc) continuous 
      infusion of GHRH (1200 mg/day) using a slow release pellet; C) the same as B plus 
      1 daily sc injection of long acting somatostatin (SS) (octreotide, 20 mg) ; D) 3 
      daily sc GHRH (250 mg) injections ; E) 2 daily sc injections of GHRH (250 mg) and 
      2 of natural SS (250 mg). In experiment 2, recently weaned male lambs were 
      continuously GHRH-treated using sc osmotic minipumps (900 mg/day) alone or 
      combined with a daily sc injection of octreotide (20 mg) for 4 weeks. Basal 
      plasma GH levels were increased after chronic pulsatile GHRH treatment but not 
      after any kind of continuous GHRH administration. This increment was maintained 
      during the 3 weeks of experimentation and appeared accompanied by a pituitary GH 
      content similar to controls. A marked GH response to the iv GHRH challenge was 
      observed in controls and in lambs receiving both types of continuous sc GHRH 
      infusions, whereas pulsatile sc GHRH-treated animals did not respond to the iv 
      GHRH challenge in the first and second weeks of the study but did so in the third 
      week of treatment. These data demonstrate that long-term pulsatile GHRH 
      administration is capable of stimulating GH release in growing male lambs, 
      without producing pituitary desensitization.
FAU - Perez-Romero, A
AU  - Perez-Romero A
AD  - Departamentos de Fisiologia, Facultad de Medicina, Universidad Complutense de 
      Madrid, Spain.
FAU - Rol De Lama, M A
AU  - Rol De Lama MA
FAU - Granados, B
AU  - Granados B
FAU - Vara, E
AU  - Vara E
FAU - Vazquez Gonzalez, I
AU  - Vazquez Gonzalez I
FAU - Ariznavarreta, C
AU  - Ariznavarreta C
FAU - Tresguerres, J A
AU  - Tresguerres JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Spain
TA  - J Physiol Biochem
JT  - Journal of physiology and biochemistry
JID - 9812509
RN  - 51110-01-1 (Somatostatin)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 9002-72-6 (Growth Hormone)
RN  - 9034-39-3 (Growth Hormone-Releasing Hormone)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Drug Administration Routes
MH  - Drug Administration Schedule
MH  - Growth Hormone/blood/*metabolism
MH  - Growth Hormone-Releasing Hormone/*administration & dosage/blood/pharmacology
MH  - Hypothalamus/metabolism
MH  - Insulin-Like Growth Factor I/analysis
MH  - Male
MH  - Pituitary Gland/drug effects/*metabolism
MH  - Random Allocation
MH  - Sheep
MH  - Somatostatin/metabolism/pharmacology
MH  - Time Factors
MH  - Weight Gain/drug effects
EDAT- 2000/10/03 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/03 11:00
PHST- 2000/10/03 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/03 11:00 [entrez]
AID - 10.1007/BF03179906 [doi]
PST - ppublish
SO  - J Physiol Biochem. 2000 Jun;56(2):107-15. doi: 10.1007/BF03179906.