PMID- 11014828
OWN - NLM
STAT- MEDLINE
DCOM- 20001130
LR  - 20210915
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 156
IP  - 2
DP  - 2000 Oct
TI  - Comparative fluorescence in situ hybridization mapping of a 431-kb Arabidopsis 
      thaliana bacterial artificial chromosome contig reveals the role of chromosomal 
      duplications in the expansion of the Brassica rapa genome.
PG  - 833-8
AB  - Comparative genome studies are important contributors to our understanding of 
      genome evolution. Most comparative genome studies in plants have been based on 
      genetic mapping of homologous DNA loci in different genomes. Large-scale 
      comparative physical mapping has been hindered by the lack of efficient and 
      affordable techniques. We report here the adaptation of fluorescence in situ 
      hybridization (FISH) techniques for comparative physical mapping between 
      Arabidopsis thaliana and Brassica rapa. A set of six bacterial artificial 
      chromosomes (BACs) representing a 431-kb contiguous region of chromosome 2 of A. 
      thaliana was mapped on both chromosomes and DNA fibers of B. rapa. This DNA 
      fragment has a single location in the A. thaliana genome, but hybridized to four 
      to six B. rapa chromosomes, indicating multiple duplications in the B. rapa 
      genome. The sizes of the fiber-FISH signals from the same BACs were not longer in 
      B. rapa than those in A. thaliana, suggesting that this genomic region is 
      duplicated but not expanded in the B. rapa genome. The comparative fiber-FISH 
      mapping results support that chromosomal duplications, rather than regional 
      expansion due to accumulation of repetitive sequences in the intergenic regions, 
      played the major role in the evolution of the B. rapa genome.
FAU - Jackson, S A
AU  - Jackson SA
AD  - Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706, 
      USA.
FAU - Cheng, Z
AU  - Cheng Z
FAU - Wang, M L
AU  - Wang ML
FAU - Goodman, H M
AU  - Goodman HM
FAU - Jiang, J
AU  - Jiang J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
SB  - IM
MH  - Arabidopsis/*genetics
MH  - Brassica/*genetics
MH  - *Chromosome Mapping
MH  - *Chromosomes, Artificial, Bacterial
MH  - *Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - *Ploidies
PMC - PMC1461289
EDAT- 2000/10/03 11:00
MHDA- 2001/02/28 10:01
PMCR- 2001/01/01
CRDT- 2000/10/03 11:00
PHST- 2000/10/03 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/03 11:00 [entrez]
PHST- 2001/01/01 00:00 [pmc-release]
AID - 10.1093/genetics/156.2.833 [doi]
PST - ppublish
SO  - Genetics. 2000 Oct;156(2):833-8. doi: 10.1093/genetics/156.2.833.