PMID- 11016686
OWN - NLM
STAT- MEDLINE
DCOM- 20010111
LR  - 20190915
IS  - 0904-2512 (Print)
IS  - 0904-2512 (Linking)
VI  - 29
IP  - 9
DP  - 2000 Oct
TI  - Oral and skin keratinocytes are stimulated to secrete monocyte chemoattractant 
      protein-1 by tumour necrosis factor-alpha and interferon-gamma.
PG  - 438-44
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a member of the CC subfamily of 
      chemokines. It is chemoattractant for monocytes, activated memory T cells and 
      dendritic cells. We studied MCP-1 mRNA and protein production in cultured human 
      oral keratinocytes (OK) and skin keratinocytes (SK). MCP-1 production was 
      undetectable in resting keratinocytes. However, stimulation with tumour necrosis 
      factors (TNF-alpha) or interferon-gamma (IFN-gamma) induced MCP-1 mRNA and 
      protein synthesis in both cell types. Together, TNF-alpha and IFN-gamma acted 
      synergistically to increase MCP-1 production. In each case MCP-1 production was 
      greater for SK than OK. The kinetics of IFN-gamma-induced MCP-1 production were 
      similar for both cell types, peaking around 72 h. In contrast, TNF-alpha induced 
      a more rapid increase in MCP-1 production by SK than OK, peak production 
      occurring after 24 and 72 h, respectively. IL-1alpha and IL-4 did not induce 
      MCP-1 production. However, in combination with IFN-gamma, they induced a small 
      extra increase in MCP-1 production in SK but not OK.
FAU - Li, J
AU  - Li J
AD  - Oral Disease Research Centre, St. Bartholomew's and the Royal London School of 
      Medicine and Dentistry, Queen Mary and Westfield College, UK.
FAU - Farthing, P M
AU  - Farthing PM
FAU - Thornhill, M H
AU  - Thornhill MH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - J Oral Pathol Med
JT  - Journal of oral pathology & medicine : official publication of the International 
      Association of Oral Pathologists and the American Academy of Oral Pathology
JID - 8911934
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis
MH  - Chemotaxis, Leukocyte/drug effects/physiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Interferon-gamma/pharmacology/*physiology
MH  - Keratinocytes/drug effects/immunology/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Mouth Mucosa/cytology/*immunology
MH  - RNA, Messenger/analysis/biosynthesis
MH  - Recombinant Proteins/pharmacology
MH  - Skin/cytology/immunology
MH  - Tumor Necrosis Factor-alpha/*physiology
EDAT- 2000/10/04 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/04 11:00
PHST- 2000/10/04 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/04 11:00 [entrez]
AID - 10.1034/j.1600-0714.2000.290904.x [doi]
PST - ppublish
SO  - J Oral Pathol Med. 2000 Oct;29(9):438-44. doi: 10.1034/j.1600-0714.2000.290904.x.