PMID- 11018762
OWN - NLM
STAT- MEDLINE
DCOM- 20001109
LR  - 20190709
IS  - 0022-0795 (Print)
IS  - 0022-0795 (Linking)
VI  - 167
IP  - 1
DP  - 2000 Oct
TI  - Alternative 5'-untranslated regions of mouse GH receptor/binding protein 
      messenger RNA are derived from sequences adjacent to the major L2 promoter.
PG  - 145-52
AB  - Heterogeneity of 5' untranslated region (5'UTR) sequences is a common feature of 
      growth hormone receptor/binding protein (GHR/BP) mRNA from a number of species. 
      Two major 5'UTR sequences (designated L1 and L2 in the mouse) have been cloned 
      from rodents, ruminants and primates, and are known to correspond to transcripts 
      generated from independently regulated promoters. A variable number of other 
      5'UTRs with diverse sequences have been cloned from rat, human and bovine 
      tissues. To characterize alternative 5'UTR usage in mouse GHR/BP mRNA, we carried 
      out 5' rapid amplification of cDNA ends using RNA from non-pregnant mouse liver 
      and adipose tissue. Three novel 5'UTR sequences were obtained. Sequencing of 
      genomic DNA revealed that exons corresponding to these three sequences are 
      clustered within 1 kb downstream of the exon encoding 5'UTR L2, and the 
      associated L2 promoter. The novel 5'UTRs are present at very low levels relative 
      to the total pool of GHR/BP mRNA in liver, fat, kidney, and mammary gland as 
      determined by ribonuclease protection assays. On the basis of these data, we 
      propose that these 5'UTR sequences may result from the use of cryptic 
      transcription start sites and splice donor sites under the influence of the 
      adjacent L2 promoter/enhancer region.
FAU - Moffat, J G
AU  - Moffat JG
AD  - Department of Biology, University of California Santa Cruz, Santa Cruz, 
      California 95064, USA.
FAU - Dao, H
AU  - Dao H
FAU - Talamantes, F
AU  - Talamantes F
LA  - eng
GR  - CA71590/CA/NCI NIH HHS/United States
GR  - GM08312/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Somatotropin)
RN  - 0 (Untranslated Regions)
RN  - W06KFL3RDT (somatotropin-binding protein)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Carrier Proteins/*genetics
MH  - Cattle
MH  - DNA, Complementary/genetics
MH  - Exons/genetics
MH  - Female
MH  - Gene Expression
MH  - Genome
MH  - Mice
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic/*genetics
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Receptors, Somatotropin/*genetics
MH  - Untranslated Regions/*genetics/metabolism
EDAT- 2000/10/06 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/06 11:00
PHST- 2000/10/06 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/06 11:00 [entrez]
AID - JOE03804 [pii]
AID - 10.1677/joe.0.1670145 [doi]
PST - ppublish
SO  - J Endocrinol. 2000 Oct;167(1):145-52. doi: 10.1677/joe.0.1670145.