PMID- 11020545
OWN - NLM
STAT- MEDLINE
DCOM- 20010109
LR  - 20190831
IS  - 0165-3806 (Print)
IS  - 0165-3806 (Linking)
VI  - 123
IP  - 1
DP  - 2000 Sep 30
TI  - Exogenous BDNF, NT-3 and NT-4 differentially regulate neurite outgrowth in 
      cultured hippocampal neurons.
PG  - 1-11
AB  - Multiple growth factors contribute to the differentiation of dendritic and axonal 
      processes by a neuron. Cultured hippocampal cells elaborate dendritic and axonal 
      processes following well-defined steps. We used this culture system to determine 
      the specific effects of brain-derived neurotrophic factor (BDNF), neurotrophin-3 
      (NT-3) and neurotrophin-4 (NT-4) on dendritic and axonal differentiation in 
      hippocampal pyramidal neurons. We demonstrated that each of these neurotrophins 
      exert distinct effects on neurite outgrowth. Both BDNF and NT-3 had positive 
      effects on the outgrowth of undifferentiated neurites, called minor neurites, and 
      on the axonal process of hippocampal pyramidal neurons. However, the effect of 
      NT-3 was more important than that of BDNF. On the other hand, NT-4 did not 
      enhance axonal outgrowth but had only an effect on the outgrowth of minor 
      neurites. Since cytoskeletal proteins play crucial roles in promoting neurite 
      outgrowth, we examined the protein levels of some of these proteins that are 
      associated with neurite outgrowth: beta-actin, gamma-actin, alpha-tubulin, MAP2 
      and tau. Surprisingly, we did not detect any change in their protein levels. 
      Taken together, our results show that BDNF, NT-3 and NT-4 exert distinct effects 
      on the neuritic compartments of hippocampal neurons.
FAU - Labelle, C
AU  - Labelle C
AD  - Departement de Pathologie et Biologie Cellulaire, Universite de Montreal, C.P. 
      6128, Succ. Centre-ville, H3C 3J7, Quebec, Canada.
FAU - Leclerc, N
AU  - Leclerc N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Dev Brain Res
JT  - Brain research. Developmental brain research
JID - 8908639
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Axons/drug effects/ultrastructure
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Count
MH  - Cells, Cultured
MH  - Cytoskeletal Proteins/metabolism
MH  - Hippocampus/cytology/*drug effects/ultrastructure
MH  - Immunohistochemistry
MH  - Nerve Growth Factors/*pharmacology
MH  - Neurites/*drug effects/metabolism/ultrastructure
MH  - Neurons/*drug effects/metabolism/ultrastructure
MH  - Neurotrophin 3/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2000/10/06 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/06 11:00
PHST- 2000/10/06 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/06 11:00 [entrez]
AID - S0165380600000699 [pii]
AID - 10.1016/s0165-3806(00)00069-9 [doi]
PST - ppublish
SO  - Brain Res Dev Brain Res. 2000 Sep 30;123(1):1-11. doi: 
      10.1016/s0165-3806(00)00069-9.