PMID- 11020861
OWN - NLM
STAT- MEDLINE
DCOM- 20010208
LR  - 20181130
IS  - 0172-6390 (Print)
IS  - 0172-6390 (Linking)
VI  - 47
IP  - 34
DP  - 2000 Jul-Aug
TI  - Production and characterization of immortalized rat hepatocytes secreting 
      hepatocyte growth factor/scatter factor.
PG  - 978-83
AB  - BACKGROUND/AIMS: Hepatocyte transplantation is a recently attractive field in the 
      treatment of liver failure and enzyme-deficient diseases. However, procurement of 
      sufficient quantities of hepatocytes is almost impossible. We attempted to create 
      a hepatocyte cell line that could be used for hepatocyte transplantation. 
      METHODOLOGY: L2A2 is a conditionally immortalized rat hepatocyte cell line 
      produced by transfection of temperature-sensitive simian virus T antigen to the 
      hepatocytes in the Lewis rat. Hepatocyte Growth Factor/Scatter Factor 
      (HGF/SF)-secreting L2A2 cells, designated as SF-21, was produced by transfecting 
      human HGF/SF cDNA into L2A2 cells. RESULTS: This cell line was able to produce 
      HGF/SF at the rate of 5-10 ng/10(6) cells/24 hrs, and the recombinant HGF/SF was 
      of the expected size and was functionally active in that it could scatter 
      Madin-Darby canine kidney cells. The SF-21 cells grew faster than its parental 
      cell clone, and survived and proliferated at 37 degrees C in vitro. Also, the 
      SF-21 cells were able to survive and proliferate when transplanted into the 
      spleen of syngeneic rat, and expressed glucose-6-phosphatase. CONCLUSIONS: These 
      HGF/SF-secreting hepatocytes can be used as a model system to test a feasibility 
      of using genetically engineered hepatocyte cell line for hepatocyte 
      transplantation in the rat.
FAU - Lee, J H
AU  - Lee JH
AD  - Department of Biochemistry, Ajou University School of Medicine, Suwon, Korea.
FAU - Kim, W H
AU  - Kim WH
FAU - Park, H
AU  - Park H
FAU - Yun, C
AU  - Yun C
FAU - Kim, B H
AU  - Kim BH
FAU - Kwak, S J
AU  - Kwak SJ
FAU - Cho, H
AU  - Cho H
FAU - Kim, M W
AU  - Kim MW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Hepatogastroenterology
JT  - Hepato-gastroenterology
JID - 8007849
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Culture Techniques/*methods
MH  - Cell Line
MH  - Hepatocyte Growth Factor/*metabolism
MH  - Hepatocytes/*metabolism/transplantation
MH  - Immunohistochemistry
MH  - Male
MH  - Rats
MH  - Rats, Inbred Lew/genetics
MH  - Transfection
EDAT- 2000/10/06 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/10/06 11:00
PHST- 2000/10/06 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/10/06 11:00 [entrez]
PST - ppublish
SO  - Hepatogastroenterology. 2000 Jul-Aug;47(34):978-83.