PMID- 11021822
OWN - NLM
STAT- MEDLINE
DCOM- 20001025
LR  - 20240413
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 157
IP  - 4
DP  - 2000 Oct
TI  - Novel protective effects of stem cell factor in a murine model of acute septic 
      peritonitis. Dependence on MCP-1.
PG  - 1177-86
AB  - Mast cells participate in the host response during sepsis and have been shown to 
      have a protective effect in a murine model of acute septic peritonitis and 
      multi-organ failure initiated by cecal ligation and puncture (CLP). Stem cell 
      factor (SCF) is a hematopoietic cytokine important in mast cell proliferation and 
      activation. In the present study, we examined the protective effects of a single 
      intraperitoneal injection of SCF given 2 hours before CLP surgery in mice. Four 
      days after the CLP surgery, SCF pretreatment significantly improved mouse 
      survival from 29 to 56% and mast cells were absolutely required for this effect. 
      Immunoneutralization studies revealed that the SCF-stimulated release of monocyte 
      chemoattractant protein-1 (MCP-1) into the septic peritoneal cavity contributed 
      to the protective effect of SCF in this model. One potential cellular source of 
      MCP-1 was the SCF-activated mast cell. In addition, SCF pretreatment 
      significantly augmented circulating levels of SCF and the immunomodulatory 
      cytokine interleukin-10 in septic mice, in part because the SCF pretreatment 
      seemed to promote the release of both mediators from the liver. Additional 
      hepatic effects of SCF treatment included an accelerated expression of hepatic 
      levels of signal transducer and activator of transcription-3 (STAT-3) in CLP mice 
      pretreated with SCF. Taken together, the findings from the present study 
      demonstrate that the intraperitoneal delivery of SCF has a major protective 
      effect in a murine model of CLP.
FAU - Bone-Larson, C L
AU  - Bone-Larson CL
AD  - Department of Pathology, University of Michigan Medical School, Ann Arbor, 
      Michigan. University of California, Los Angeles, California, USA.
FAU - Hogaboam, C M
AU  - Hogaboam CM
FAU - Steinhauser, M L
AU  - Steinhauser ML
FAU - Oliveira, S H
AU  - Oliveira SH
FAU - Lukacs, N W
AU  - Lukacs NW
FAU - Strieter, R M
AU  - Strieter RM
FAU - Kunkel, S L
AU  - Kunkel SL
LA  - eng
GR  - R01 HL031237/HL/NHLBI NIH HHS/United States
GR  - HL31237/HL/NHLBI NIH HHS/United States
GR  - HL60289/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Chemokine CCL2)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (Stat3 protein, mouse)
RN  - 0 (Stem Cell Factor)
RN  - 0 (Trans-Activators)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Cecum
MH  - Cell Nucleus/metabolism
MH  - Chemokine CCL2/metabolism
MH  - DNA-Binding Proteins/metabolism
MH  - Female
MH  - Interleukin-10/metabolism
MH  - Ligation
MH  - Liver/drug effects/metabolism
MH  - Mast Cells/drug effects/pathology/physiology
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Peritonitis/*metabolism/mortality/*pathology
MH  - Punctures
MH  - STAT3 Transcription Factor
MH  - Stem Cell Factor/blood/metabolism/*pharmacology
MH  - Therapeutic Irrigation
MH  - Trans-Activators/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC1850153
EDAT- 2000/10/06 11:00
MHDA- 2001/02/28 10:01
PMCR- 2001/04/01
CRDT- 2000/10/06 11:00
PHST- 2000/10/06 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/06 11:00 [entrez]
PHST- 2001/04/01 00:00 [pmc-release]
AID - S0002-9440(10)64633-0 [pii]
AID - 2372 [pii]
AID - 10.1016/S0002-9440(10)64633-0 [doi]
PST - ppublish
SO  - Am J Pathol. 2000 Oct;157(4):1177-86. doi: 10.1016/S0002-9440(10)64633-0.