PMID- 11024130
OWN - NLM
STAT- MEDLINE
DCOM- 20001120
LR  - 20190508
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 74
IP  - 21
DP  - 2000 Nov
TI  - Incoming human cytomegalovirus pp65 (UL83) contained in apoptotic infected 
      fibroblasts is cross-presented to CD8(+) T cells by dendritic cells.
PG  - 10018-24
AB  - Human cytomegalovirus (HCMV) infection is well controlled mainly by cytotoxic 
      CD8(+) T lymphocytes (CTL) directed against the matrix protein pp65 despite the 
      numerous immune escape mechanisms developed by the virus. Dendritic cells (DCs) 
      are key antigen-presenting cells for the generation of an immune response which 
      have the capacity to acquire antigens via endocytosis of apoptotic cells and thus 
      present peptides to major histocompatibility complex class I-restricted T cells. 
      We examined whether this mechanism could contribute to the activation of 
      anti-pp65 CTL. In this study, we show that infection by HCMV AD169 induced 
      sensitization of MRC5 fibroblasts to tumor necrosis factor alpha-mediated 
      apoptosis very early after virus inoculation and that pp65 contained in apoptotic 
      cells came from the delivery of the matrix protein into the cell. We observed 
      that immature DCs derived from peripheral monocytes were not permissive to HCMV 
      AD169 infection but were able to internalize pp65-positive apoptotic infected 
      MRC5 cells. We then demonstrated that following exposure to these apoptotic 
      bodies, DCs could activate HLA-A2- or HLA-B35-restricted anti-pp65 CTL, 
      suggesting that they acquired and processed properly fibroblast-derived pp65. 
      Together, our data suggest that cross-presentation of incoming pp65 contained in 
      apoptotic cells may provide a quick and efficient way to prime anti-HCMV CD8(+) T 
      cells.
FAU - Arrode, G
AU  - Arrode G
AD  - INSERM U395, IFR 30, UPS, CNRS, CHU, 31024 Toulouse Cedex, France.
FAU - Boccaccio, C
AU  - Boccaccio C
FAU - Lule, J
AU  - Lule J
FAU - Allart, S
AU  - Allart S
FAU - Moinard, N
AU  - Moinard N
FAU - Abastado, J P
AU  - Abastado JP
FAU - Alam, A
AU  - Alam A
FAU - Davrinche, C
AU  - Davrinche C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Phosphoproteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (cytomegalovirus matrix protein 65kDa)
SB  - IM
MH  - *Antigen Presentation
MH  - Antigen-Presenting Cells
MH  - Apoptosis
MH  - Cell Line
MH  - Cytomegalovirus/*immunology
MH  - Dendritic Cells/*immunology
MH  - Fibroblasts/*virology
MH  - Humans
MH  - Lymphocyte Activation
MH  - Phosphoproteins/*immunology
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Viral Matrix Proteins/*immunology
PMC - PMC102040
EDAT- 2000/10/12 11:00
MHDA- 2001/02/28 10:01
PMCR- 2000/11/01
CRDT- 2000/10/12 11:00
PHST- 2000/10/12 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/12 11:00 [entrez]
PHST- 2000/11/01 00:00 [pmc-release]
AID - 0711 [pii]
AID - 10.1128/jvi.74.21.10018-10024.2000 [doi]
PST - ppublish
SO  - J Virol. 2000 Nov;74(21):10018-24. doi: 10.1128/jvi.74.21.10018-10024.2000.