PMID- 11025361
OWN - NLM
STAT- MEDLINE
DCOM- 20010111
LR  - 20181130
IS  - 0012-2823 (Print)
IS  - 0012-2823 (Linking)
VI  - 62
IP  - 2-3
DP  - 2000
TI  - Gastrin-mediated alterations in gastric epithelial apoptosis and proliferation in 
      a mastomys rodent model of gastric neoplasia.
PG  - 143-51
AB  - BACKGROUND/AIMS: Hypergastrinemia secondary to low acid secretion is associated 
      with gastric carcinoid formation in Mastomys. We investigated the effect of 
      gastrin on oxyntic epithelial apoptosis and proliferation in this model. METHODS: 
      Hypergastrinemia and mucosal hyperplasia were induced by irreversible H(2) 
      receptor blockade with loxtidine. Gastrin levels were normalised in some animals 
      by 10 days' loxtidine withdrawal. Serum gastrin was determined by 
      radioimmunoassay, proliferative, enterochromaffin-like cells and Bcl-2 protein 
      family expression by immunohistochemistry, and apoptotic cells by terminal 
      deoxyuridine nucleotide nick end labeling (TUNEL). RESULTS: Proliferating cells 
      were increased 4-fold in loxtidine-treated animals, and returned to normal upon 
      loxtidine withdrawal. Enterochromaffin-like cell number increased 2-fold with 
      loxtidine, and did not decrease after withdrawal. Apoptotic epithelial cells were 
      located at the luminal surface and increased 1.8-fold with loxtidine, returning 
      to control levels upon withdrawal. The ratio of proliferative to apoptotic cells 
      was lower in the control and withdrawn groups than in the loxtidine group 
      (0.26+/-0.05 and 0.26+/-0.08 vs. 0.77+/-0.12). With hypergastrinemia, the 
      expression of Bcl-2 and Bak was increased and Bax decreased in the middle of the 
      gland. CONCLUSION: Hypergastrinemia is associated with alterations in both 
      proliferation and apoptosis in Mastomys gastric mucosa. This may contribute to 
      the pathogenesis of mucosal hyperplasia in this model.
CI  - Copyright 2000 S. Karger AG, Basel.
FAU - Kidd, M
AU  - Kidd M
AD  - Gastrointestinal Clinic, UCT Medical School Observatory, Cape Town, South Africa.
FAU - Tang, L H
AU  - Tang LH
FAU - Modlin, I M
AU  - Modlin IM
FAU - Zhang, T
AU  - Zhang T
FAU - Chin, K
AU  - Chin K
FAU - Holt, P R
AU  - Holt PR
FAU - Moss, S F
AU  - Moss SF
LA  - eng
GR  - R29/DK48820/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Digestion
JT  - Digestion
JID - 0150472
RN  - 0 (Gastrins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Carcinoid Tumor/*etiology/pathology
MH  - Cell Division/drug effects
MH  - Disease Models, Animal
MH  - Female
MH  - Gastric Acid/metabolism
MH  - Gastric Mucosa/*physiology
MH  - Gastrins/*analysis/pharmacokinetics/pharmacology
MH  - Immunohistochemistry
MH  - Male
MH  - Muridae
MH  - Proto-Oncogene Proteins c-bcl-2/genetics
MH  - Stomach Neoplasms/*etiology/pathology
EDAT- 2000/10/12 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/12 11:00
PHST- 2000/10/12 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/12 11:00 [entrez]
AID - 7806 [pii]
AID - 10.1159/000007806 [doi]
PST - ppublish
SO  - Digestion. 2000;62(2-3):143-51. doi: 10.1159/000007806.