PMID- 11025408
OWN - NLM
STAT- MEDLINE
DCOM- 20001121
LR  - 20171101
IS  - 0028-3835 (Print)
IS  - 0028-3835 (Linking)
VI  - 72
IP  - 3
DP  - 2000 Sep
TI  - Brain-derived neurotrophic factor but not neurotrophin-3 enhances differentiation 
      of somatostatin neurons in hypothalamic cultures.
PG  - 144-53
AB  - The present work investigated whether neurotrophins could differentially affect 
      in vitro growth and maturation of two related subsets of hypothalamic neurons, 
      hypophysiotropic somatostatin (SRIH) neurons projecting from the periventricular 
      area and arcuate SRIH interneurons. For this purpose, the hypothalamus of 
      17-day-old rat fetuses was sampled and separated into a ventral and a dorsal 
      fragment containing respectively periventricular and arcuate regions. Each 
      fragment was dissociated and seeded separately in defined medium. Brain-derived 
      neurotrophic factor (BDNF) or neurotrophin-3 (NT-3), two important members of the 
      neurotrophin family involved in neuronal differentiation and plasticity, were 
      added to the cultures at seeding time. After 6 or 11 days in vitro, neurons were 
      labeled with an anti-SRIH antiserum and submitted to morphometric analysis. In 
      parallel, SRIH mRNA was estimated by semiquantitative 
      reverse-transcriptase-polymerase chain reaction, and neuronal SRIH content, basal 
      and depolarisation-stimulated releases measured by radioimmunoassay. The response 
      of control, non-labeled neurons was estimated by neuronal counts and by assaying 
      glutamic acid decarboxylase, a marker of a large majority of hypothalamic 
      neurons. BDNF markedly increased the size and the branching number of SRIH 
      periventricular cell bodies. Expression of SRIH mRNA, as well as SRIH content and 
      release into the culture medium, were also stimulated by the neurotrophin. 
      Non-SRIH neurons were not affected by the treatment. Under the same conditions, 
      arcuate neurons exhibited a weak, mostly transient response to BDNF. NT-3 was 
      ineffective on either neuronal subset. Immunoneutralization of Trk receptors 
      provided further evidence for BDNF effect specificity. The results indicate that 
      BDNF is a selective activator of the differentiation of hypophysiotropic SRIH 
      neurons in the periventricular area of the hypothalamus.
CI  - Copyright 2000 S. Karger AG, Basel.
FAU - Loudes, C
AU  - Loudes C
AD  - INSERM U159, Paris, France. loudes@broca.inserm.fr
FAU - Petit, F
AU  - Petit F
FAU - Kordon, C
AU  - Kordon C
FAU - Faivre-Bauman, A
AU  - Faivre-Bauman A
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Neuroendocrinology
JT  - Neuroendocrinology
JID - 0035665
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
RN  - 51110-01-1 (Somatostatin)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.10.1 (Receptor, trkC)
SB  - IM
MH  - Animals
MH  - Arcuate Nucleus of Hypothalamus/cytology/drug effects/embryology/metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism/pharmacology
MH  - Cell Differentiation/drug effects/*physiology
MH  - Cells, Cultured/cytology/drug effects/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Fetus
MH  - Hypothalamus/cytology/*drug effects/embryology/*metabolism
MH  - Neurons/cytology/*drug effects/metabolism
MH  - Neurotrophin 3/*metabolism/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/immunology/metabolism
MH  - Receptor, trkC/immunology/metabolism
MH  - Somatostatin/*metabolism
EDAT- 2000/10/12 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/12 11:00
PHST- 2000/10/12 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/12 11:00 [entrez]
AID - 54581 [pii]
AID - 10.1159/000054581 [doi]
PST - ppublish
SO  - Neuroendocrinology. 2000 Sep;72(3):144-53. doi: 10.1159/000054581.