PMID- 11027401
OWN - NLM
STAT- MEDLINE
DCOM- 20001101
LR  - 20190906
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 426
IP  - 4
DP  - 2000 Oct 30
TI  - Differential regulation of trophic factor receptor mRNAs in spinal motoneurons 
      after sciatic nerve transection and ventral root avulsion in the rat.
PG  - 587-601
AB  - After sciatic nerve lesion in the adult rat, motoneurons survive and regenerate, 
      whereas the same lesion in the neonatal animal or an avulsion of ventral roots 
      from the spinal cord in adults induces extensive cell death among lesioned 
      motoneurons with limited or no axon regeneration. A number of substances with 
      neurotrophic effects have been shown to increase survival of motoneurons in vivo 
      and in vitro. Here we have used semiquantitative in situ hybridization 
      histochemistry to detect the regulation in motoneurons of mRNAs for receptors to 
      ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), glial cell 
      line-derived neurotrophic factor (GDNF), brain-derived neurotrophic factor 
      (BDNF), and neurotrophin-3 (NT-3) 1-42 days after the described three types of 
      axon injury. After all types of injury, the mRNAs for GDNF receptors (GFRalpha-1 
      and c-RET) and the LIF receptor LIFR were distinctly (up to 300%) up-regulated in 
      motoneurons. The CNTF receptor CNTFRalpha mRNA displayed only small changes, 
      whereas the mRNA for membrane glycoprotein 130 (gp130), which is a critical 
      receptor component for LIF and CNTF transduction, was profoundly down-regulated 
      in motoneurons after ventral root avulsion. The BDNF full-length receptor trkB 
      mRNA was up-regulated acutely after adult sciatic nerve lesion, whereas after 
      ventral root avulsion trkB was down-regulated. The NT-3 receptor trkC mRNA was 
      strongly down-regulated after ventral root avulsion. The results demonstrate that 
      removal of peripheral nerve tissue from proximally lesioned motor axons induces 
      profound down-regulations of mRNAs for critical components of receptors for CNTF, 
      LIF, and NT-3 in affected motoneurons, but GDNF receptor mRNAs are up-regulated 
      in the same situation. These results should be considered in relation to the 
      extensive cell death among motoneurons after ventral root avulsion and should 
      also be important for the design of therapeutical approaches in cases of 
      motoneuron death.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Hammarberg, H
AU  - Hammarberg H
AD  - Department of Neuroscience, Nobels v. 12A, Karolinska Institute, S-171 77 
      Stockholm, Sweden. Henrik.Hammarberg@neuro.ki.se
FAU - Piehl, F
AU  - Piehl F
FAU - Risling, M
AU  - Risling M
FAU - Cullheim, S
AU  - Cullheim S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Growth Factor)
SB  - IM
MH  - Aging/metabolism
MH  - Animals
MH  - Animals, Newborn/growth & development/metabolism/physiology
MH  - *Axotomy/methods
MH  - Cell Survival/physiology
MH  - Denervation
MH  - Motor Neurons/*metabolism
MH  - Nerve Regeneration/physiology
MH  - RNA, Messenger/*metabolism
MH  - Rats/*metabolism
MH  - Rats, Sprague-Dawley
MH  - Receptors, Growth Factor/*genetics
MH  - Reference Values
MH  - Sciatic Nerve/physiology
MH  - Spinal Cord/cytology/*metabolism
MH  - Spinal Nerve Roots/injuries/physiology
MH  - Up-Regulation
MH  - Wounds and Injuries/metabolism
EDAT- 2000/10/12 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/12 11:00
PHST- 2000/10/12 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/12 11:00 [entrez]
AID - 10.1002/1096-9861(20001030)426:4<587::AID-CNE7>3.0.CO;2-R [pii]
AID - 10.1002/1096-9861(20001030)426:4<587::aid-cne7>3.0.co;2-r [doi]
PST - ppublish
SO  - J Comp Neurol. 2000 Oct 30;426(4):587-601. doi: 
      10.1002/1096-9861(20001030)426:4<587::aid-cne7>3.0.co;2-r.