PMID- 11027427
OWN - NLM
STAT- MEDLINE
DCOM- 20001207
LR  - 20181113
IS  - 0007-0920 (Print)
IS  - 1532-1827 (Electronic)
IS  - 0007-0920 (Linking)
VI  - 83
IP  - 9
DP  - 2000 Nov
TI  - Hepatocyte growth factor/scatter factor enhances the invasion of mesothelioma 
      cell lines and the expression of matrix metalloproteinases.
PG  - 1147-53
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a multifunctional factor 
      involved both in development and tissue repair, as well as pathological processes 
      such as cancer and metastasis. It has been identified in vivo in many types of 
      tumours together with its tyrosine kinase receptor, Met. We show here that 
      exogenous HGF/SF acts as a strong chemoattractant for human mesothelioma cell 
      lines. The factor also enhanced cell adhesion to and invasion into Matrigel. The 
      mesothelioma cell lines synthesized a panel of matrix metalloproteinases critical 
      for tumour progression such as MMP-1, 2, 3, 9 and membrane-bound MT1-MMP. HGF/SF 
      stimulated the expression of MMP-1, 9 and MT1-MMP and had a slight effect on 
      expression of the MMP inhibitor TIMP-1 but not TIMP-2. However, there was no 
      simple correlation between the levels of MMPs and TIMPs of the cell lines and 
      their different invasion properties or between HGF/SF stimulatory effects on MMP 
      expression and invasion. In addition, effects of protease inhibitors on invasion 
      suggested that serine proteases were also expressed in human mesothelioma cell 
      lines and were involved in HGF/SF-induced invasion. The results show a 
      predominant role for HGF/SF in mesothelioma cell invasion, stimulating 
      simultaneously adhesion, motility, invasion and regulation of MMP and TIMP 
      levels.
CI  - Copyright 2000 Cancer Research Campaign.
FAU - Harvey, P
AU  - Harvey P
AD  - School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK.
FAU - Clark, I M
AU  - Clark IM
FAU - Jaurand, M C
AU  - Jaurand MC
FAU - Warn, R M
AU  - Warn RM
FAU - Edwards, D R
AU  - Edwards DR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Cancer
JT  - British journal of cancer
JID - 0370635
RN  - 0 (Drug Combinations)
RN  - 0 (Laminin)
RN  - 0 (Proteoglycans)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 119978-18-6 (matrigel)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Cell Adhesion/drug effects
MH  - Cell Movement/*drug effects
MH  - Collagen
MH  - Dose-Response Relationship, Drug
MH  - Drug Combinations
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Humans
MH  - Laminin
MH  - Matrix Metalloproteinase 1/drug effects/metabolism
MH  - Matrix Metalloproteinase 2/drug effects/metabolism
MH  - Matrix Metalloproteinase 9/drug effects/metabolism
MH  - Matrix Metalloproteinases/*drug effects/metabolism
MH  - Mesothelioma/*enzymology/pathology
MH  - Neoplasm Invasiveness
MH  - Proteoglycans
MH  - Tissue Inhibitor of Metalloproteinase-1/drug effects/metabolism
MH  - Tumor Cells, Cultured
PMC - PMC2363594
EDAT- 2000/10/12 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/12 11:00
PHST- 2000/10/12 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/12 11:00 [entrez]
AID - S0007092000914459 [pii]
AID - 10.1054/bjoc.2000.1445 [doi]
PST - ppublish
SO  - Br J Cancer. 2000 Nov;83(9):1147-53. doi: 10.1054/bjoc.2000.1445.