PMID- 11028668
OWN - NLM
STAT- MEDLINE
DCOM- 20010208
LR  - 20190822
IS  - 0903-1936 (Print)
IS  - 0903-1936 (Linking)
VI  - 16
IP  - 3
DP  - 2000 Sep
TI  - Enhanced peripheral leukocyte leukotriene production and bronchial 
      hyperresponsiveness in asthmatics.
PG  - 504-8
AB  - Leukotrienes (LTs) are pro-inflammatory mediators that contribute to the 
      pathophysiological features of asthma. The relationship between the amounts of 
      LTB4 and LTC4 produced by the leukocytes of asthmatic patients on the one hand 
      and immunoglobulin E (IgE)-mediated allergy, asthma exacerbations and bronchial 
      hyperresponsiveness was studied. Leukocytes were obtained from peripheral blood 
      drawn from 29 atopic and 27 nonatopic asthmatics during exacerbations and 
      clinically controlled periods, as well as from 20 control individuals. The 
      leukocytes were stimulated with calcium ionophore A23187 to induce LTB4 and LTC4 
      production. Allergy was assessed by means of specific serum IgE or by positive 
      skin tests, whereas bronchial hyperresponsiveness was measured by methacholine 
      challenge. The leukocytes of the asthmatics generated significantly more LTB4 
      (p<0.05) and LTC4 (p<0.01) than those of controls. The leukocytes of patients 
      with atopic asthma generated significantly more LTC4 than those of patients with 
      nonatopic asthma (p<0.01). Significantly more LTC4 was produced by leukocytes 
      obtained during exacerbations, than by those obtained during clinically 
      controlled periods (p<0.01). In addition, there was a significant correlation 
      between LTB4 generation by leukocytes and the degree of bronchial 
      hyperresponsiveness to methacholine (r=-0.792, p<0.0001). These results suggest 
      that leukotriene C4 production by leukocytes is associated with immunoglobulin 
      E-mediated allergy and asthma exacerbations, and further that generation of 
      leukotriene B4 is closely related to bronchial hyperresponsiveness in patients 
      with asthma.
FAU - Mitsunobu, F
AU  - Mitsunobu F
AD  - Dept of Medicine, Misasa Medical Branch, Okayama University Medical School, 
      Tottori, Japan.
FAU - Mifune, T
AU  - Mifune T
FAU - Hosaki, Y
AU  - Hosaki Y
FAU - Ashida, K
AU  - Ashida K
FAU - Tsugeno, H
AU  - Tsugeno H
FAU - Okamoto, M
AU  - Okamoto M
FAU - Harada, S
AU  - Harada S
FAU - Takata, S
AU  - Takata S
FAU - Tanizaki, Y
AU  - Tanizaki Y
FAU - Harada, M
AU  - Harada M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Eur Respir J
JT  - The European respiratory journal
JID - 8803460
RN  - 1HGW4DR56D (Leukotriene B4)
RN  - 2CU6TT9V48 (Leukotriene C4)
SB  - IM
MH  - Asthma/immunology/*physiopathology
MH  - Bronchial Hyperreactivity/*physiopathology
MH  - Female
MH  - Humans
MH  - Leukocytes/*metabolism
MH  - Leukotriene B4/*biosynthesis
MH  - Leukotriene C4/*biosynthesis
MH  - Male
MH  - Middle Aged
EDAT- 2000/10/12 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/10/12 11:00
PHST- 2000/10/12 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/10/12 11:00 [entrez]
AID - 10.1034/j.1399-3003.2000.016003504.x [doi]
PST - ppublish
SO  - Eur Respir J. 2000 Sep;16(3):504-8. doi: 10.1034/j.1399-3003.2000.016003504.x.