PMID- 11029614
OWN - NLM
STAT- MEDLINE
DCOM- 20001130
LR  - 20190815
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 12
IP  - 10
DP  - 2000 Oct
TI  - Expression of c-Met in developing rat hippocampus: evidence for HGF as a 
      neurotrophic factor for calbindin D-expressing neurons.
PG  - 3453-61
AB  - Hepatocyte growth factor-scatter factor (HGF) is expressed in different parts of 
      the nervous system, and has been shown to exhibit neurotrophic activity. Here we 
      show that c-Met, the receptor for HGF, is expressed in developing rat 
      hippocampus, with the highest levels during the first postnatal weeks. To study 
      the function of HGF, hippocampal neurons were prepared from embryonic rats and 
      treated with different HGF concentrations. In these cultures, HGF increased the 
      number of neurons expressing the 28-kDa calcium-binding protein (calbindin D) in 
      a dose-dependent manner. The effect of HGF was larger than that observed with 
      either brain-derived neurotrophic factor (BDNF) or neurotrophin-3 (NT-3), and 
      cotreatment of the cultures with HGF and the neurotrophins was additive with 
      respect to calbindin D neurons. Besides affecting the number of neurons, HGF 
      significantly increased the degree of sprouting of calbindin D-positive neurons, 
      suggesting an influence on neuronal maturation. BDNF and NT-3 stimulated neurite 
      outgrowth of calbindin D neurons to a much smaller degree. In contrast to 
      calbindin D neurons, HGF did not significantly increase the number of neurons 
      immunoreactive with the neurotransmitter gamma-aminobutyric acid (GABA) in the 
      hippocampal cultures. Immunohistochemical studies showed that c-Met-, calbindin 
      D- and HGF-immunoreactive cells are all present in the dentate gyrus and partly 
      colocalize within neurons. These results show that HGF acts on calbindin 
      D-containing hippocampal neurons and increases their neurite outgrowth, 
      suggesting that HGF plays an important role for the maturation and function of 
      these neurons in the hippocampus.
FAU - Korhonen, L
AU  - Korhonen L
AD  - Department of Neuroscience Neurobiology, Uppsala University, Box 587, BMC, 
      S-75123 Uppsala, Sweden.
FAU - Sjoholm, U
AU  - Sjoholm U
FAU - Takei, N
AU  - Takei N
FAU - Kern, M A
AU  - Kern MA
FAU - Schirmacher, P
AU  - Schirmacher P
FAU - Castren, E
AU  - Castren E
FAU - Lindholm, D
AU  - Lindholm D
LA  - eng
PT  - Journal Article
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Calbindins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 0 (RNA, Messenger)
RN  - 0 (S100 Calcium Binding Protein G)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/metabolism/pharmacology
MH  - Calbindins
MH  - Cell Count
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Drug Administration Schedule
MH  - Fetus
MH  - Hepatocyte Growth Factor/*metabolism/pharmacology
MH  - Hippocampus/cytology/drug effects/*embryology/*metabolism
MH  - Nerve Growth Factors/*metabolism
MH  - Neurites/drug effects/metabolism/ultrastructure
MH  - Neurons/cytology/drug effects/*metabolism
MH  - Neurotrophin 3/metabolism/pharmacology
MH  - Proto-Oncogene Proteins c-met/genetics/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - S100 Calcium Binding Protein G/*metabolism
MH  - Time Factors
MH  - gamma-Aminobutyric Acid/metabolism
EDAT- 2000/10/13 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/13 11:00
PHST- 2000/10/13 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/13 11:00 [entrez]
AID - ejn260 [pii]
AID - 10.1046/j.1460-9568.2000.00260.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2000 Oct;12(10):3453-61. doi: 10.1046/j.1460-9568.2000.00260.x.