PMID- 11031082
OWN - NLM
STAT- MEDLINE
DCOM- 20001207
LR  - 20061115
IS  - 0014-4886 (Print)
IS  - 0014-4886 (Linking)
VI  - 166
IP  - 1
DP  - 2000 Nov
TI  - Development of astroglial elements in the suprachiasmatic nucleus of the rat: 
      with special reference to the involvement of the optic nerve.
PG  - 44-51
AB  - The development of astroglial cells and the effect of the retinohypothalamic 
      tract on it were studied by vimentin and glial fibrillary acidic protein (GFAP) 
      immunocytochemistry in the suprachiasmatic nucleus (SCN) of the rat. At the 
      embryonic stage, vimentin-immunoreactive (VIM-IR) radial glia, precursors of 
      astrocytes, were dominant. However, their filaments vanished in the first few 
      postnatal days. Instead of VIM-IR glial filaments, GFAP-immunoreactive (GFAP-IR) 
      astrocytes appeared at E20 and grew rapidly from the P3 stage. GFAP 
      immunoreactivity in the ventrolateral portion of the SCN (VLSCN) was measured 
      using a computer-assisted image analyzing system. In normal rats, GFAP 
      immunoreactivity showed a stepwise pattern with two slopes at P3-P4 and P20-P25. 
      Bilaterally eye-enucleated rats operated on the day of birth showed lower GFAP 
      immunoreactivity than normal rats and the GFAP immunoreactivity did not increase 
      between P20 and P25 when GFAP-IR glial processes rapidly expand. Electron 
      microscopic investigation at P50 (adult stage) revealed that neurons in the VLSCN 
      had often direct apposition without astroglial processes and the frequency of 
      this finding was significantly higher in eye-enucleated rats than in the control 
      rats. These findings strongly suggest that the postnatal development of 
      astroglial elements, particularly the expansion of GFAP-IR processes in the SCN, 
      is regulated by retinohypothalamic projection.
CI  - Copyright 2000 Academic Press.
FAU - Munekawa, K
AU  - Munekawa K
AD  - Department of Anatomy and Neurobiology, Kyoto Prefectural University of Medicine, 
      Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto, 602-0841, Japan.
FAU - Tamada, Y
AU  - Tamada Y
FAU - Iijima, N
AU  - Iijima N
FAU - Hayashi, S
AU  - Hayashi S
FAU - Ishihara, A
AU  - Ishihara A
FAU - Inoue, K
AU  - Inoue K
FAU - Tanaka, M
AU  - Tanaka M
FAU - Ibata, Y
AU  - Ibata Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Vimentin)
SB  - IM
MH  - Afferent Pathways/embryology/*growth & development/ultrastructure
MH  - Age Factors
MH  - Animals
MH  - Animals, Newborn
MH  - Astrocytes/*metabolism/ultrastructure
MH  - Cell Communication/*physiology
MH  - Cell Differentiation/*physiology
MH  - Eye Enucleation/adverse effects
MH  - Female
MH  - Fetus
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Male
MH  - Optic Nerve/embryology/*growth & development/ultrastructure
MH  - Pregnancy
MH  - Rats
MH  - Retina/*embryology/*growth & development/ultrastructure
MH  - Suprachiasmatic Nucleus/embryology/*growth & development/ultrastructure
MH  - Vimentin/metabolism
EDAT- 2000/10/14 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/14 11:00
PHST- 2000/10/14 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/14 11:00 [entrez]
AID - S0014-4886(00)97490-9 [pii]
AID - 10.1006/exnr.2000.7490 [doi]
PST - ppublish
SO  - Exp Neurol. 2000 Nov;166(1):44-51. doi: 10.1006/exnr.2000.7490.