PMID- 11032903
OWN - NLM
STAT- MEDLINE
DCOM- 20001109
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 75
IP  - 5
DP  - 2000 Nov
TI  - Coordinated expression of beta-amyloid precursor protein and the putative 
      beta-secretase BACE and alpha-secretase ADAM10 in mouse and human brain.
PG  - 2133-43
AB  - To define the enzymes involved in the etiology of Alzheimer's disease, we 
      compared in mouse and human brain the mRNA levels and cellular localization of 
      the ubiquitous beta-amyloid precursor protein (beta-APP) with those of the 
      putative alpha-secretases ADAM10 and ADAM17 and the beta-secretases BACE and 
      BACE2. In situ hybridization performed in mice during prenatal and postnatal 
      development and in adulthood revealed the coexpression of beta-APP, BACE, and 
      ADAM10. The patterns of BACE2 and ADAM17 only partially overlapped with that of 
      beta-APP. beta-APP, BACE, and ADAM10 mRNAs have also been detected by northern 
      blot in human brain cortex of normal subjects and in Alzheimer's disease 
      subjects. In situ hybridization performed using combined biotin- and radiolabeled 
      riboprobes provided evidence for the coexpression of beta-APP with BACE and 
      ADAM10 in human cortical neurons. Our data provide cytochemical evidence 
      supporting the role of ADAM10 and BACE as authentic alpha- and beta-secretases.
FAU - Marcinkiewicz, M
AU  - Marcinkiewicz M
AD  - Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of 
      Montreal, Montreal, Quebec, Canada. Marcinm@ircm.qc.ca
FAU - Seidah, N G
AU  - Seidah NG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.- (Amyloid Precursor Protein Secretases)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 3.4.23.- (Aspartic Acid Endopeptidases)
RN  - EC 3.4.23.45 (BACE2 protein, human)
RN  - EC 3.4.23.46 (BACE1 protein, human)
RN  - EC 3.4.23.46 (Bace1 protein, mouse)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.81 (ADAM10 Protein)
RN  - EC 3.4.24.81 (ADAM10 protein, human)
RN  - EC 3.4.24.81 (Adam10 protein, mouse)
RN  - EC 3.4.24.86 (ADAM17 Protein)
RN  - EC 3.4.24.86 (ADAM17 protein, human)
RN  - EC 3.4.24.86 (Adam17 protein, mouse)
SB  - IM
MH  - ADAM Proteins
MH  - ADAM10 Protein
MH  - ADAM17 Protein
MH  - Aged
MH  - Alzheimer Disease/metabolism
MH  - Amyloid Precursor Protein Secretases
MH  - Amyloid beta-Protein Precursor/*biosynthesis/genetics
MH  - Animals
MH  - Aspartic Acid Endopeptidases/*biosynthesis/genetics
MH  - Autoradiography
MH  - Blotting, Northern
MH  - Brain/embryology/*metabolism/pathology
MH  - Endopeptidases/*biosynthesis/genetics
MH  - Frontal Lobe/metabolism
MH  - Gene Expression
MH  - Glycoproteins/biosynthesis/genetics
MH  - Humans
MH  - In Situ Hybridization
MH  - Membrane Proteins/*biosynthesis/genetics
MH  - Metalloendopeptidases/*biosynthesis/genetics/metabolism
MH  - Mice
MH  - Middle Aged
MH  - Neurons/cytology/metabolism
MH  - Parietal Lobe/metabolism
MH  - RNA, Messenger/metabolism
EDAT- 2000/10/18 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/18 11:00
PHST- 2000/10/18 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/18 11:00 [entrez]
AID - 10.1046/j.1471-4159.2000.0752133.x [doi]
PST - ppublish
SO  - J Neurochem. 2000 Nov;75(5):2133-43. doi: 10.1046/j.1471-4159.2000.0752133.x.