PMID- 11034212
OWN - NLM
STAT- MEDLINE
DCOM- 20001019
LR  - 20131121
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 407
IP  - 6804
DP  - 2000 Oct 5
TI  - Skin abnormalities generated by temporally controlled RXRalpha mutations in mouse 
      epidermis.
PG  - 633-6
AB  - Nuclear receptors for retinoids (RARs) and vitamin D (VDR), and for some other 
      ligands (TRs, PPARs and LXRs), maybe critical in the development and homeostasis 
      of mammalian epidermis. It is believed that these receptors form heterodimers 
      with retinoid X receptors (RXRs) to act as transcriptional regulators. However, 
      most genetic approaches aimed at establishing their physiological functions in 
      the skin have been inconclusive owing either to pleiotropic effects and 
      redundancies between receptor isotypes in gene knockouts, or to equivocal 
      interpretation of dominant-negative mutant studies in transgenic mice. Moreover, 
      knockout of RXRalpha, the main skin RXR isotype, is lethal in utero before skin 
      formation. Here we have resolved these problems by developing an efficient 
      technique to create spatiotemporally controlled somatic mutations in the mouse. 
      We used tamoxifen-inducible Cre-ER(T) recombinases to ablate RXRalpha selectively 
      in adult mouse keratinocytes. We show that RXRalpha has key roles in hair 
      cycling, probably through RXR/VDR heterodimers, and in epidermal keratinocyte 
      proliferation and differentiation.
FAU - Li, M
AU  - Li M
AD  - Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS/INSERM/ULP, 
      College de France, Illkirch.
FAU - Indra, A K
AU  - Indra AK
FAU - Warot, X
AU  - Warot X
FAU - Brocard, J
AU  - Brocard J
FAU - Messaddeq, N
AU  - Messaddeq N
FAU - Kato, S
AU  - Kato S
FAU - Metzger, D
AU  - Metzger D
FAU - Chambon, P
AU  - Chambon P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Transcription Factors)
RN  - 0 (Viral Proteins)
RN  - 094ZI81Y45 (Tamoxifen)
RN  - EC 2.7.7.- (Cre recombinase)
RN  - EC 2.7.7.- (Integrases)
SB  - IM
MH  - Alleles
MH  - Alopecia/etiology/genetics/pathology
MH  - Animals
MH  - Female
MH  - Hair/physiology
MH  - Humans
MH  - Integrases/biosynthesis
MH  - Keratinocytes/physiology
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - Mutagenesis
MH  - Receptors, Retinoic Acid/genetics/*physiology
MH  - Retinoid X Receptors
MH  - Skin Diseases/*etiology/genetics/pathology
MH  - *Skin Physiological Phenomena
MH  - Tamoxifen
MH  - Transcription Factors/genetics/*physiology
MH  - *Viral Proteins
EDAT- 2000/10/18 11:00
MHDA- 2000/10/21 11:01
CRDT- 2000/10/18 11:00
PHST- 2000/10/18 11:00 [pubmed]
PHST- 2000/10/21 11:01 [medline]
PHST- 2000/10/18 11:00 [entrez]
AID - 10.1038/35036595 [doi]
PST - ppublish
SO  - Nature. 2000 Oct 5;407(6804):633-6. doi: 10.1038/35036595.