PMID- 11035052
OWN - NLM
STAT- MEDLINE
DCOM- 20001114
LR  - 20200914
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 165
IP  - 8
DP  - 2000 Oct 15
TI  - A subset of cytolytic dendritic cells in rat.
PG  - 4202-8
AB  - Dendritic cells (DCs) are a rare population of leukocytes specialized in Ag 
      processing and presentation to T cells. We have previously shown that cultured 
      rat splenic DCs exhibit a cytotoxic activity against selected target cells. In 
      this study, we analyzed this function in DCs freshly prepared from lymphoid 
      organs using the DC-specific OX62 mAb and magnetic beads. Freshly extracted 
      splenic DCs, but not lymph node and thymic DCs, exhibited a strong and moderate 
      cytotoxic activity against YAC-1 and K562 target cells, respectively. FACS 
      analyses showed that spleen contained a minor subset (10-15%) of CD4(+) and class 
      II(int) DCs that also expressed the OX41 Ag and the lymphoid-related Ags CD5 and 
      CD90 (Thy-1) and a major (80-85%) subset of CD4(-)/OX41(-)/CD5(-) and class 
      II(int) DCs. The cytotoxic activity of splenic DCs was strictly restricted to the 
      CD4(-) DCs, a subset poorly represented in LN and thymus. Contrasting with our 
      previous report using cultured splenic DCs, freshly isolated splenic DCs killed 
      YAC-1 cells using a Ca(2+)-independent mechanism, but this function did not 
      appear mediated by Fas ligand, TNF-related apoptosis-inducing ligand, or 
      TNF-alpha. Therefore, rat DCs contain a subset of naturally cytolytic cells that 
      could play a role in both innate and acquired immune responses. Together with our 
      previous report, these data suggest that rat DCs can use two mechanisms of 
      cytotoxicity depending on their maturation/activation state.
FAU - Trinite, B
AU  - Trinite B
AD  - Institut National de la Sante et de la Recherche Medicale Unite 437 and Institut 
      de Transplantation et de Recherche en Transplantation, Nantes, France.
FAU - Voisine, C
AU  - Voisine C
FAU - Yagita, H
AU  - Yagita H
FAU - Josien, R
AU  - Josien R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (CD4 Antigens)
RN  - 0 (Fas Ligand Protein)
RN  - 0 (Fasl protein, mouse)
RN  - 0 (Faslg protein, rat)
RN  - 0 (Ligands)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Pore Forming Cytotoxic Proteins)
RN  - 0 (TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (Tnfsf10 protein, mouse)
RN  - 0 (Tnfsf10 protein, rat)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (fas Receptor)
RN  - 126465-35-8 (Perforin)
SB  - IM
MH  - Animals
MH  - Apoptosis/immunology
MH  - Apoptosis Regulatory Proteins
MH  - CD4 Antigens/biosynthesis
MH  - Cell Line
MH  - Cell Separation
MH  - *Cytotoxicity, Immunologic
MH  - Dendritic Cells/*immunology/metabolism
MH  - Fas Ligand Protein
MH  - Immunophenotyping
MH  - Killer Cells, Natural/immunology
MH  - Leukemia L5178
MH  - Ligands
MH  - Lymph Nodes/cytology/immunology/metabolism
MH  - Membrane Glycoproteins/physiology
MH  - Mice
MH  - Organ Specificity/immunology
MH  - Perforin
MH  - Pore Forming Cytotoxic Proteins
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Rats, Sprague-Dawley
MH  - Spleen/cytology/immunology/metabolism
MH  - TNF-Related Apoptosis-Inducing Ligand
MH  - Thymus Gland/cytology/immunology/metabolism
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/physiology
MH  - fas Receptor/physiology
EDAT- 2000/10/18 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/18 11:00
PHST- 2000/10/18 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/18 11:00 [entrez]
AID - 10.4049/jimmunol.165.8.4202 [doi]
PST - ppublish
SO  - J Immunol. 2000 Oct 15;165(8):4202-8. doi: 10.4049/jimmunol.165.8.4202.