PMID- 11035713 OWN - NLM STAT- MEDLINE DCOM- 20001115 LR - 20210526 IS - 0019-9567 (Print) IS - 1098-5522 (Electronic) IS - 0019-9567 (Linking) VI - 68 IP - 11 DP - 2000 Nov TI - Chemokine C10 promotes disease resolution and survival in an experimental model of bacterial sepsis. PG - 6108-14 AB - Previous studies have suggested that the C-C chemokine C10 is involved in the chronic stages of host defense reactions. The present study addressed the role of C10 in a murine model of septic peritonitis, induced by cecal ligation and puncture (CLP). Unlike other C-C chemokines, C10 levels in the peritoneal wash were increased approximately 30-fold above baseline levels at 48 h after CLP surgery. Immunoneutralization of peritoneal C10 levels with polyclonal anti-C10 antiserum during CLP-induced peritonitis negatively impacted mouse survival over 4 days. In contrast, when 500 ng of recombinant murine C10 was administered immediately after CLP surgery, the 4-day survival rate increased from 20% to over 60%. The C10 therapy appeared to facilitate a rapid and significant enhancement of the levels of tumor necrosis factor alpha (TNF-alpha) and monocyte chemoattractant protein-1 (MCP-1) and a later increase in interleukin-13 (IL-13) levels in the peritoneal cavity. In vitro studies showed that the combination of IL-1beta and C10 markedly augmented TNF-alpha synthesis by peritoneal macrophages and that C10 synthesis was induced in these cells following their exposure to IL-13. At 24 h after CLP surgery, only 25% of C10-treated mice were bacteremic versus 85% of the control group that exhibited dissemination of bacteria into the circulation. The lack of bacteremia in C10-treated mice appeared to be related, in part, to in vitro evidence that C10 significantly enhanced the bacterial phagocytic activity of peritoneal macrophages. In addition, in vivo evidence suggested that C10 therapy significantly reduced the amount of material that leaked from the damaged gut. Taken together, the results of this study demonstrate that the C10 chemokine rapidly promotes disease resolution in the CLP model through its direct effects on the cellular events critically involved in host defense during septic peritonitis. FAU - Steinhauser, M L AU - Steinhauser ML AD - Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109-0602, USA. FAU - Hogaboam, C M AU - Hogaboam CM FAU - Matsukawa, A AU - Matsukawa A FAU - Lukacs, N W AU - Lukacs NW FAU - Strieter, R M AU - Strieter RM FAU - Kunkel, S L AU - Kunkel SL LA - eng GR - IP50HL56402/HL/NHLBI NIH HHS/United States GR - R01 AI036302/AI/NIAID NIH HHS/United States GR - P50 HL056402/HL/NHLBI NIH HHS/United States GR - P01 HL031963/HL/NHLBI NIH HHS/United States GR - R37 HL035276/HL/NHLBI NIH HHS/United States GR - HL31963/HL/NHLBI NIH HHS/United States GR - R29 AI036302/AI/NIAID NIH HHS/United States GR - HL35276/HL/NHLBI NIH HHS/United States PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Infect Immun JT - Infection and immunity JID - 0246127 RN - 0 (Chemokine CCL2) RN - 0 (Chemokines, CC) RN - 0 (Immune Sera) RN - 0 (Interleukin-1) RN - 0 (Interleukin-13) RN - 0 (Recombinant Proteins) RN - 0 (Tumor Necrosis Factor-alpha) SB - IM MH - Animals MH - Bacteremia/*immunology/mortality/therapy MH - Chemokine CCL2/biosynthesis MH - Chemokines, CC/*physiology/therapeutic use MH - Female MH - Immune Sera/immunology MH - Interleukin-1/pharmacology MH - Interleukin-13/biosynthesis/pharmacology MH - Mice MH - Phagocytosis MH - Rabbits MH - Recombinant Proteins/therapeutic use MH - Tumor Necrosis Factor-alpha/biosynthesis PMC - PMC97687 EDAT- 2000/10/18 11:00 MHDA- 2001/02/28 10:01 PMCR- 2000/11/01 CRDT- 2000/10/18 11:00 PHST- 2000/10/18 11:00 [pubmed] PHST- 2001/02/28 10:01 [medline] PHST- 2000/10/18 11:00 [entrez] PHST- 2000/11/01 00:00 [pmc-release] AID - 0282 [pii] AID - 10.1128/IAI.68.11.6108-6114.2000 [doi] PST - ppublish SO - Infect Immun. 2000 Nov;68(11):6108-14. doi: 10.1128/IAI.68.11.6108-6114.2000.