PMID- 11035721
OWN - NLM
STAT- MEDLINE
DCOM- 20001115
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 68
IP  - 11
DP  - 2000 Nov
TI  - Detection and characterization of autoagglutination activity by Campylobacter 
      jejuni.
PG  - 6168-75
AB  - In several gram-negative bacterial pathogens, autoagglutination (AAG) activity is 
      a marker for interaction with host cells and virulence. Campylobacter jejuni 
      strains also show AAG, but this property varies considerably among strains. To 
      examine the characteristics of C. jejuni AAG, we developed a quantitative in 
      vitro assay. For strain 81-176, which shows high AAG, activity was optimal for 
      cells grown for < or = 24 h, was independent of growth temperature, and was best 
      measured for cells suspended in phosphate-buffered saline at 25 degrees C for 24 
      h. AAG activity was heat labile and was abolished by pronase or acid-glycine (pH 
      2.2) treatment but not by lipase, DNase, or sodium metaperiodate. Strain 4182 has 
      low AAG activity, but extraction with water increased AAG, suggesting the loss of 
      an inhibitor. Strain 6960 has weak AAG with no effect due to water extraction. 
      Our study with clinical isolates suggests that C. jejuni strains may be grouped 
      into three AAG phenotypes. A variant derived from strain 81116 that is flagellate 
      but immotile showed the strong AAG exhibited by the parent strain, suggesting 
      that motility per se is not necessary for the AAG activity. AAG correlated with 
      both bacterial hydrophobicity and adherence to INT407 cells. Mutants which lack 
      flagella (flaA, flaB, and flbA) or common cell surface antigen (peb1A) were 
      constructed in strain 81-176 by natural transformation-mediated allelic exchange. 
      Both AAG activity and bacterial hydrophobicity were abolished in the aflagellate 
      mutants but not the peb1A mutant. In total, these findings indicate that C. 
      jejuni AAG is highly associated with flagellar expression.
FAU - Misawa, N
AU  - Misawa N
AD  - Division of Infectious Diseases, Vanderbilt University School of Medicine, A-3310 
      Medical Center North, Nashville, Tennessee 37232, USA. 
      a0d901u@cc.miyazaki-u.ac.jp
FAU - Blaser, M J
AU  - Blaser MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
SB  - IM
MH  - *Agglutination
MH  - Bacterial Adhesion
MH  - Campylobacter jejuni/*pathogenicity
MH  - Cell Line
MH  - Flagella/physiology
MH  - Humans
PMC - PMC97695
EDAT- 2000/10/18 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/18 11:00
PHST- 2000/10/18 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/18 11:00 [entrez]
AID - 0424 [pii]
AID - 10.1128/IAI.68.11.6168-6175.2000 [doi]
PST - ppublish
SO  - Infect Immun. 2000 Nov;68(11):6168-75. doi: 10.1128/IAI.68.11.6168-6175.2000.