PMID- 11037968
OWN - NLM
STAT- MEDLINE
DCOM- 20001103
LR  - 20181130
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 68
IP  - 4
DP  - 2000 Oct
TI  - Functional consequences of FcepsilonRIalpha up-regulation by IgE in human 
      basophils.
PG  - 479-86
AB  - These studies examine the functional changes that occur after up-regulation of 
      FcepsilonRIalpha by immunoglobulin E (IgE) for human basophils. Basophils were 
      cultured with and without IgE antibody (PS myeloma IgE or anti-gp120-specific 
      IgE) for 1 week and challenged with anti-IgE, anti-FcepsilonRIalpha, or antigen 
      for histamine and IL-4 secretion. There were no statistically significant changes 
      in their response to anti-IgE or anti-receptor antibodies, as compared with 
      controls incubated for the same period, whereas receptor expression increased an 
      average of 4-fold. There was increased responsiveness to antigenic challenge, 
      most notably at suboptimal concentrations of antigen (gp120 peptide-ovalbumin 
      conjugate). For a 6-fold difference in cell surface density of gp120-specific 
      IgE, there was a 2.2-fold change in antigen potency or 3-fold increases in 
      histamine release at lower antigen concentrations. Similar results were found for 
      secretion of IL-4. Basophil sensitivity, which is a measure of the density of 
      antigen-specific IgE required for 50% of maximal secretion, was used to determine 
      whether up-regulation of FcepsilonRIalpha was coordinated with up-regulation of 
      other components of the IgE-signaling pathway. The results indicated 
      up-regulation of FcepsilonRI is not always accompanied by changes that allow 
      sensitivity to be maintained. These results indicate that functional 
      up-regulation does occur but that its magnitude may be modulated because not all 
      components of the signaling pathway are up-regulated in a balanced manner.
FAU - MacGlashan, D Jr
AU  - MacGlashan D Jr
AD  - Johns Hopkins Asthma and Allergy Center, Baltimore, Maryland 21224, USA. 
      dmacglas@welch.jhu.edu
FAU - Schroeder, J T
AU  - Schroeder JT
LA  - eng
GR  - A142220/PHS HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Interleukin-13)
RN  - 0 (Receptors, IgE)
RN  - 0 (anti-IgE antibodies)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Antibodies, Anti-Idiotypic/pharmacology
MH  - Basophils/immunology/*metabolism
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Immunologic
MH  - Flow Cytometry
MH  - Humans
MH  - Immunoglobulin E/*pharmacology
MH  - Interleukin-13/metabolism
MH  - Interleukin-4/metabolism
MH  - Receptors, IgE/*biosynthesis/genetics
MH  - Up-Regulation/*drug effects
EDAT- 2000/10/19 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/19 11:00
PHST- 2000/10/19 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/19 11:00 [entrez]
PST - ppublish
SO  - J Leukoc Biol. 2000 Oct;68(4):479-86.