PMID- 11037974
OWN - NLM
STAT- MEDLINE
DCOM- 20001103
LR  - 20051117
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 68
IP  - 4
DP  - 2000 Oct
TI  - Escherichia coli cytotoxic necrotizing factor-1 (CNF-1) increases the adherence 
      to epithelia and the oxidative burst of human polymorphonuclear leukocytes but 
      decreases bacteria phagocytosis.
PG  - 522-8
AB  - Recruitment of polymorphonuclear leukocytes (PMNL) is a hallmark of both urinary 
      and digestive infections caused by Escherichia coli. Cytotoxic necrotizing factor 
      1 (CNF-1) is a toxin produced by uropathogenic E. coli strains that mediates its 
      effects via the activation of small GTP-binding proteins. However, the role and 
      the consequences of CNF-1 on PMNL physiology remain largely unknown. In this 
      study, we provide evidence that CNF-1 dramatically affects the PMNL cytoskeleton 
      architecture by inducing an increased content of F-actin. Furthermore, we 
      demonstrate that CNF-1 increases functional features of PMNL, such as superoxide 
      generation and adherence on epithelial T84 monolayers, but significantly 
      decreases their phagocytic function. Our results suggest that CNF-1 may behave as 
      a virulence factor in urinary or digestive infection by stimulating PMNL 
      cytotoxicity as a result of its enhancing effect on their adherence to epithelial 
      cells as well as the production of radical oxygen products. Moreover, the 
      decreased phagocytosis of PMNL induced by CNF-1 likely facilitates growth of 
      bacteria. In these conditions, CNF-1 would intervene in the initiation and in the 
      perpetuation of the inflammatory process.
FAU - Hofman, P
AU  - Hofman P
AD  - Laboratoire d'Anatomie-Pathologique, INSERM U364, Nice, France. hofman@unice.fr
FAU - Le Negrate, G
AU  - Le Negrate G
FAU - Mograbi, B
AU  - Mograbi B
FAU - Hofman, V
AU  - Hofman V
FAU - Brest, P
AU  - Brest P
FAU - Alliana-Schmid, A
AU  - Alliana-Schmid A
FAU - Flatau, G
AU  - Flatau G
FAU - Boquet, P
AU  - Boquet P
FAU - Rossi, B
AU  - Rossi B
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Actins)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Cytotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Macrophage-1 Antigen)
RN  - 0 (Opsonin Proteins)
RN  - 0 (Reactive Oxygen Species)
RN  - 106803-33-2 (cytotoxic necrotizing factor type 1)
RN  - 11062-77-4 (Superoxides)
RN  - 9010-72-4 (Zymosan)
RN  - EC 3.6.5.2 (rho GTP-Binding Proteins)
SB  - IM
MH  - Actins/metabolism
MH  - Bacterial Toxins/*pharmacology
MH  - Cell Adhesion/drug effects
MH  - Cells, Cultured
MH  - Cytoskeleton/drug effects
MH  - Cytotoxins/*pharmacology
MH  - Epithelial Cells/cytology
MH  - Escherichia coli/*pathogenicity
MH  - *Escherichia coli Proteins
MH  - Humans
MH  - Inflammation/chemically induced
MH  - Intestinal Mucosa/cytology
MH  - Macrophage-1 Antigen/metabolism
MH  - Neutrophils/*drug effects/microbiology/physiology/ultrastructure
MH  - Opsonin Proteins/pharmacology
MH  - Phagocytosis/*drug effects
MH  - Reactive Oxygen Species/metabolism
MH  - Respiratory Burst/*drug effects
MH  - Superoxides/metabolism
MH  - Virulence
MH  - Zymosan/pharmacology
MH  - rho GTP-Binding Proteins/antagonists & inhibitors/physiology
EDAT- 2000/10/19 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/19 11:00
PHST- 2000/10/19 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/19 11:00 [entrez]
PST - ppublish
SO  - J Leukoc Biol. 2000 Oct;68(4):522-8.