PMID- 11037977
OWN - NLM
STAT- MEDLINE
DCOM- 20001103
LR  - 20061115
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 68
IP  - 4
DP  - 2000 Oct
TI  - Monocyte:astrocyte interactions regulate MCP-1 expression in both cell types.
PG  - 545-52
AB  - As astrocytes are a source of monocyte chemoattractant protein-1 (MCP-1) and lie 
      in close apposition to brain microvessels, interactions between astrocytes and 
      infiltrating monocytes might regulate production of this chemokine. To 
      investigate this possibility, a monocyte:astrocyte co-culture model was utilized 
      to assess the respective roles of these two cell types in regulating MCP-1 
      production. Results indicate that, while neither monocytes nor astrocytes alone 
      produce detectable levels of MCP-1, co-culture of these two cell types results in 
      time-dependent production of this chemokine. Such production requires de novo 
      protein synthesis and is dependent on physical contact between monocytes and 
      astrocytes, involving engagement of the cell-adhesion molecules ICAM-1 and 
      VCAM-1. Additionally, interleukin 1-beta (IL-1beta) and tumor necrosis 
      factor-alpha (TNF-alpha) are soluble mediators of this response. These findings 
      imply that monocyte extravasation into the CNS may be critically regulated at the 
      blood-brain barrier by specialized monocyte:astrocyte interactions.
FAU - Andjelkovic, A V
AU  - Andjelkovic AV
AD  - Department of Pharmacology, University of Connecticut Health Center, Farmington 
      06030, USA.
FAU - Kerkovich, D
AU  - Kerkovich D
FAU - Pachter, J S
AU  - Pachter JS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Astrocytes/*cytology/metabolism/ultrastructure
MH  - Cell Adhesion
MH  - *Cell Communication
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Coculture Techniques
MH  - Gene Expression Regulation/*physiology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/physiology
MH  - Interleukin-1/physiology
MH  - Monocytes/*cytology/metabolism
MH  - Tumor Necrosis Factor-alpha/physiology
MH  - Vascular Cell Adhesion Molecule-1/physiology
EDAT- 2000/10/19 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/19 11:00
PHST- 2000/10/19 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/19 11:00 [entrez]
PST - ppublish
SO  - J Leukoc Biol. 2000 Oct;68(4):545-52.