PMID- 11038410
OWN - NLM
STAT- MEDLINE
DCOM- 20001116
LR  - 20151119
IS  - 0958-0670 (Print)
IS  - 0958-0670 (Linking)
VI  - 85
IP  - 5
DP  - 2000 Sep
TI  - Carbohydrate ingestion prior to exercise augments the exercise-induced activation 
      of the pyruvate dehydrogenase complex in human skeletal muscle.
PG  - 581-6
AB  - This study examined the effect of pre-exercise carbohydrate (CHO) ingestion on 
      pyruvate dehydrogenase complex (PDC) activation, acetyl group availability and 
      substrate level phosphorylation (glycogenolysis and phosphocreatine (PCr) 
      hydrolysis) in human skeletal muscle during the transition from rest to 
      steady-state exercise. Seven male subjects performed two 10 min treadmill runs at 
      70 % maximum oxygen uptake (VO2,max), 1 week apart. Each subject ingested 8 ml 
      (kg body mass (BM))-1 of either a placebo solution (CON trial) or a 5.5 % CHO 
      solution (CHO trial) 10 min before each run. Muscle biopsy samples were obtained 
      from the vastus lateralis at rest and immediately after each trial. Muscle PDC 
      activity was higher at the end of exercise in the CHO trial compared with the CON 
      trial (1.78+/-0.18 and 1.27+/-0.16 mmol min(-1) (kg wet matter (WM))(-1), 
      respectively; P 0.05) and this was accompanied by lower acetylcarnitine 
      (7.1+/-1.2 and 9.1+/-1.1 mmol kg(-1) (dry matter (DM))(-1) in CHO and CON, 
      respectively; P<0.05) and citrate concentrations (0.73+/-0.05 and 0.91+/-0.10 
      mmol (kg DM)(-1) in CHO and CON, respectively; P<0.05). No difference was 
      observed between trials in the rates of muscle glycogen and PCr breakdown and 
      lactate accumulation. This is the first study to demonstrate that CHO ingestion 
      prior to exercise augments the exercise-induced activation of muscle PDC and 
      reduces acetylcarnitine accumulation during the transition from rest to 
      steady-state exercise. However, those changes did not affect the contribution of 
      substrate level phosphorylation to ATP resynthesis.
FAU - Tsintzas, K
AU  - Tsintzas K
AD  - Human Muscle Metabolism Research Group, Loughborough University, UK. 
      kostas.tsintzas@nottingham.ac.uk
FAU - Williams, C
AU  - Williams C
FAU - Constantin-Teodosiu, D
AU  - Constantin-Teodosiu D
FAU - Hultman, E
AU  - Hultman E
FAU - Boobis, L
AU  - Boobis L
FAU - Greenhaff, P
AU  - Greenhaff P
LA  - eng
PT  - Clinical Trial
PT  - Controlled Clinical Trial
PT  - Journal Article
PL  - England
TA  - Exp Physiol
JT  - Experimental physiology
JID - 9002940
RN  - 0 (Blood Glucose)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Fatty Acids, Nonesterified)
RN  - 0 (Insulin)
RN  - 0 (Pyruvate Dehydrogenase Complex)
RN  - 020IUV4N33 (Phosphocreatine)
RN  - 2968PHW8QP (Citric Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 6DH1W9VH8Q (Acetylcarnitine)
RN  - 8558G7RUTR (Pyruvic Acid)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - 9005-79-2 (Glycogen)
RN  - S7UI8SM58A (Carnitine)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Acetylcarnitine/metabolism
MH  - Adenosine Triphosphate/biosynthesis/metabolism
MH  - Adult
MH  - Blood Glucose/metabolism
MH  - Carnitine/metabolism
MH  - Citric Acid/metabolism
MH  - Dietary Carbohydrates/*administration & dosage/metabolism/*pharmacology
MH  - Enzyme Activation/drug effects
MH  - Exercise/*physiology
MH  - Exercise Test
MH  - Fatty Acids, Nonesterified/blood
MH  - Glycogen/metabolism
MH  - Humans
MH  - Insulin/blood
MH  - Lactic Acid/blood/metabolism
MH  - Male
MH  - Muscle, Skeletal/drug effects/*enzymology/*metabolism
MH  - Oxidative Phosphorylation
MH  - Oxygen/metabolism
MH  - Oxygen Consumption
MH  - Phosphocreatine/metabolism
MH  - Pyruvate Dehydrogenase Complex/*metabolism
MH  - Pyruvic Acid/metabolism
EDAT- 2000/10/20 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/20 11:00
PHST- 2000/10/20 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/20 11:00 [entrez]
AID - PHY_2043 [pii]
PST - ppublish
SO  - Exp Physiol. 2000 Sep;85(5):581-6.