PMID- 11041806
OWN - NLM
STAT- MEDLINE
DCOM- 20001018
LR  - 20180213
IS  - 0042-1138 (Print)
IS  - 0042-1138 (Linking)
VI  - 64
IP  - 4
DP  - 2000
TI  - Incidence of chromosome 8,10,X and Y aneuploidies in sperm nucleus of infertile 
      men detected by FISH.
PG  - 202-8
AB  - We studied the frequency of aneuploidy in sperm nuclei of six infertile men with 
      abnormal semen profile and normal karyotype, using fluorescence in situ 
      hybridization (FISH) with DNA probes for chromosomes 8, 10, X and Y. The control 
      group consisted of four healthy fertile men with normal karyotype and semen 
      profiles. The purpose of this study was to determine whether there are 
      differences between infertile male donors and control donors for: (1) the 
      incidence of sex chromosome aneuploidy, and (2) the number of disomies for 
      chromosomes 8, and 10 cosegregating with chromosomes X and Y. FISH analysis 
      showed no significant differences of sex ratios of the sperm nuclei in and 
      between infertile and control groups. The most significant abnormalities in the 
      infertile group were clusters of sperm nuclei bearing XY and XYY. In addition, 
      the incidence of disomic sperm nuclei for chromosomes 8 and 10 consegregating 
      with sex chromosomes was not significantly different between the patient and 
      control groups, nor within them. However, the total frequency of aneuploid sperm 
      nuclei was significantly different between the infertile group and the control 
      group. We observed a significant excess of sperm nuclei bearing chromosome 10 
      along with disomy for chromosome Y (10YY). In conclusion, our results from FISH 
      analysis demonstrate a significantly increased frequency of aneuploidy for the 
      sex chromosomes in sperm nuclei from infertile men. Therefore it may be concluded 
      that infertility is a risk factor for sex chromosome aneuploidy in sperm nuclei.
FAU - Acar, H
AU  - Acar H
AD  - Department of Medical Genetics, Selcuk University, Faculty of Medicine, Konya, 
      Turkey. acarx003@tc.umn.edu
FAU - Kilinc, M
AU  - Kilinc M
FAU - Cora, T
AU  - Cora T
FAU - Aktan, M
AU  - Aktan M
FAU - Taskapu, H
AU  - Taskapu H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Urol Int
JT  - Urologia internationalis
JID - 0417373
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Cell Nucleus/*genetics
MH  - *Chromosomes, Human, Pair 10
MH  - *Chromosomes, Human, Pair 8
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Incidence
MH  - Infertility, Male/*genetics
MH  - Male
MH  - Spermatozoa/*ultrastructure
MH  - *X Chromosome
EDAT- 2000/10/21 11:00
MHDA- 2000/10/21 11:01
CRDT- 2000/10/21 11:00
PHST- 2000/10/21 11:00 [pubmed]
PHST- 2000/10/21 11:01 [medline]
PHST- 2000/10/21 11:00 [entrez]
AID - 10.1159/000030531 [doi]
PST - ppublish
SO  - Urol Int. 2000;64(4):202-8. doi: 10.1159/000030531.