PMID- 11042517
OWN - NLM
STAT- MEDLINE
DCOM- 20030623
LR  - 20190116
IS  - 1042-8194 (Print)
IS  - 1026-8022 (Linking)
VI  - 37
IP  - 5-6
DP  - 2000 May
TI  - Atypical lymphocytes in B-cell chronic lymphocytic leukemia and trisomy 12 
      studied by conventional staining combined with fluorescence in situ 
      hybridization.
PG  - 571-6
AB  - Trisomy 12 is one of the most frequent chromosomal abnormalities in B-cell 
      chronic lymphocytic leukemia (CLL), and is predominantly found in CLL with 
      atypical morphology (aCLL). It has been suggested that the atypical morphology 
      might be a feature of the abnormal trisomy 12 clone, but so far it has been 
      difficult to allocate chromosomal aberrations to individual leukemic cells 
      identified by cytomorphology. We therefore wanted to use our MGG/FISH method, 
      which combines fluorescence in situ hybridization (FISH) and standard 
      cytomorphology, to study if the trisomy 12 clone in CLL was restricted to 
      lymphocytes with atypical morphology. Peripheral blood specimens of four patients 
      with aCLL were studied using a DNA probe against the pericentromeric region of 
      chromosome 12. Trisomy 12 was found in 10-34 % of the lymphocytes. In three 
      patients, the proportion of atypical and typical lymphocytes with trisomy 12 was 
      quite comparable, and so was the percentage of atypical cells with 
      lymphoplasmacytoid morphology and those with cleaved nucleus showing trisomy 12. 
      Only one patient differed, since we found an overrepresentation of trisomy 12 
      among the atypical lymphocytes. However, this could be fully explained by the 
      diluting effect of contaminating T-cells after chemotherapy. The results of the 
      present study show that despite the strong association of trisomy 12 and atypical 
      morphology in CLL, this chromosomal abnormality is not confined to lymphocytes 
      with atypical morphology, but is also found in typical CLL cells. This supports 
      that both cell types have the same clonal origin and that different cell 
      morphology cannot be explained alone by the acquisition of an additional 
      chromosome 12.
FAU - Hjalmar, V
AU  - Hjalmar V
AD  - Division of Hematology, Department of Medicine, Karolinska Hospital, Karolinska 
      Institutet, Stockholm, Sweden. viktoria.hjalmar@kids.ki.se
FAU - Kimby, E
AU  - Kimby E
FAU - Matutes, E
AU  - Matutes E
FAU - Sundstrom, C
AU  - Sundstrom C
FAU - Wallvik, J
AU  - Wallvik J
FAU - Hast, R
AU  - Hast R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Leuk Lymphoma
JT  - Leukemia & lymphoma
JID - 9007422
RN  - 0 (Coloring Agents)
RN  - 0 (May-Grunwald Giemsa)
RN  - T42P99266K (Methylene Blue)
RN  - TDQ283MPCW (Eosine Yellowish-(YS))
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - B-Lymphocytes/*pathology
MH  - Cell Nucleus/ultrastructure
MH  - *Chromosomes, Human, Pair 12
MH  - Coloring Agents
MH  - Eosine Yellowish-(YS)
MH  - Female
MH  - Humans
MH  - Immunophenotyping
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/genetics/*pathology
MH  - Male
MH  - Methylene Blue
MH  - Middle Aged
MH  - Neoplastic Stem Cells/pathology
MH  - Staining and Labeling/*methods
MH  - *Trisomy
EDAT- 2000/10/24 00:00
MHDA- 2003/06/24 05:00
CRDT- 2000/10/24 00:00
PHST- 2000/10/24 00:00 [pubmed]
PHST- 2003/06/24 05:00 [medline]
PHST- 2000/10/24 00:00 [entrez]
AID - I308J991228 [pii]
AID - 10.3109/10428190009058509 [doi]
PST - ppublish
SO  - Leuk Lymphoma. 2000 May;37(5-6):571-6. doi: 10.3109/10428190009058509.