PMID- 11044770
OWN - NLM
STAT- MEDLINE
DCOM- 20001207
LR  - 20171101
IS  - 0006-3126 (Print)
IS  - 0006-3126 (Linking)
VI  - 78
IP  - 3
DP  - 2000 Oct
TI  - Effect of polyclonal anti-TNFalpha antibody on endotoxic shock in suckling rats.
PG  - 207-11
AB  - The aim of this study was to evaluate the effect of anti-tumor necrosis factor 
      alpha (TNFalpha) antibodies on the TNFalpha gene expression in a neonatal septic 
      shock model. Ten-day-old Sprague-Dawley rats were divided into four groups and 
      given intraperitoneal (ip) injection as follows: group 1: 0.1 ml saline; group 2: 
      0.1 mg/kg Salmonella enteritidis lipopolysaccharide (LPS); group 3: 1 mg/kg of 
      anti-TNFalpha antibodies (Ab); group 4: 0.1 mg/kg of LPS and 1 mg/kg of Ab. We 
      found that in group 2, LPS induced shock, demonstrating hypoglycemia and 
      lactacidemia (p < 0. 05) and death (81.8%). Ab decreased the mortality 
      significantly (35%) and attenuated the hypoglycemia (35 +/- 8 mg/dl in group 2 
      vs. 53 +/- 3 mg/dl in group 4) and lactacidemia (5.40 +/- 0.63 vs. 2.35 +/- 0.45 
      mmol) at 8 h in group 4 when compared to group 2. Northern blot demonstrated a 
      significant decrease in TNFalpha mRNA expression in group 4 as compared to group 
      2, at 2 h after LPS injection. We conclude that the beneficial effects of 
      anti-TNFalpha antibodies on LPS-induced shock may be due to decreased TNFalpha 
      gene expression.
CI  - Copyright 2000 S. Karger AG, Basel.
FAU - Bhola, M
AU  - Bhola M
AD  - The Ronald McDonald Children's Hospital, Loyola University of Chicago, Maywood, 
      IL 60153, USA. mbhola@luc.edu
FAU - Goto, M
AU  - Goto M
FAU - Chen, H Y
AU  - Chen HY
FAU - Myers, T F
AU  - Myers TF
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Biol Neonate
JT  - Biology of the neonate
JID - 0247551
RN  - 0 (Antibodies)
RN  - 0 (Blood Glucose)
RN  - 0 (Lactates)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Animals, Suckling/*blood
MH  - Antibodies/*pharmacology
MH  - Blood Glucose/analysis
MH  - Lactates/blood
MH  - RNA, Messenger/blood
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Shock, Septic/*blood
MH  - Tumor Necrosis Factor-alpha/genetics/*immunology
EDAT- 2000/10/25 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/25 11:00
PHST- 2000/10/25 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/25 11:00 [entrez]
AID - 14272 [pii]
AID - 10.1159/000014272 [doi]
PST - ppublish
SO  - Biol Neonate. 2000 Oct;78(3):207-11. doi: 10.1159/000014272.