PMID- 11049993
OWN - NLM
STAT- MEDLINE
DCOM- 20001211
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 96
IP  - 9
DP  - 2000 Nov 1
TI  - UTY gene codes for an HLA-B60-restricted human male-specific minor 
      histocompatibility antigen involved in stem cell graft rejection: 
      characterization of the critical polymorphic amino acid residues for T-cell 
      recognition.
PG  - 3126-32
AB  - Rejection of a graft after human leukocyte antigen (HLA)-identical stem cell 
      transplantation (SCT) can be caused by recipient's immunocompetent T lymphocytes 
      recognizing minor histocompatibility antigens on donor stem cells. During 
      rejection of a male stem cell graft by a female recipient, 2 male (H-Y)-specific 
      cytotoxic T lymphocyte (CTL) clones were isolated from peripheral blood. One CTL 
      clone recognized an HLA-A2-restricted H-Y antigen, encoded by the SMCY gene. 
      Another CTL clone recognized an HLA-B60-restricted H-Y antigen. In this study UTY 
      was identified as the gene coding for the HLA-B60-restricted H-Y antigen. The 
      UTY-derived H-Y antigen was characterized as a 10-amino acid residue peptide, 
      RESEEESVSL. Although the epitope differed by 3 amino acids from its X-homologue, 
      UTX, only 2 polymorphisms were essential for recognition by the CTL clone HLA-B60 
      HY. These results illustrate that CTLs against several H-Y antigens derived from 
      different proteins can contribute simultaneously to graft rejection after 
      HLA-identical, sex-mismatched SCT. Moreover, RESEEESVSL-specific T cells could be 
      isolated from a female HLA-B60+ patient with myelodysplastic syndrome who has 
      been treated with multiple blood transfusions, but not from control healthy 
      HLA-B60+ female donors. This may indicate that RESEEESVSL-reactive T cells are 
      more common in sensitized patients.
FAU - Vogt, M H
AU  - Vogt MH
AD  - Departments of Hematology and Immunohematology and Bloodbank, Leiden University 
      Medical Center, Leiden, The Netherlands. m.h.j.vogt@lumc.nl
FAU - Goulmy, E
AU  - Goulmy E
FAU - Kloosterboer, F M
AU  - Kloosterboer FM
FAU - Blokland, E
AU  - Blokland E
FAU - de Paus, R A
AU  - de Paus RA
FAU - Willemze, R
AU  - Willemze R
FAU - Falkenburg, J H
AU  - Falkenburg JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (H-Y Antigen)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-B60 antigen)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Proteins)
RN  - 0 (UTY protein, human)
SB  - IM
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Female
MH  - Graft Rejection/genetics/*immunology
MH  - H-Y Antigen/genetics
MH  - HLA-B Antigens/*genetics
MH  - HeLa Cells
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - Male
MH  - Minor Histocompatibility Antigens/*genetics
MH  - Molecular Sequence Data
MH  - Nuclear Proteins
MH  - Oligodeoxyribonucleotides/genetics
MH  - *Polymorphism, Genetic
MH  - Proteins/chemistry/*genetics
MH  - T-Lymphocytes/*immunology
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - Transfection
MH  - *Y Chromosome
EDAT- 2000/10/26 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/26 11:00
PHST- 2000/10/26 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/26 11:00 [entrez]
AID - S0006-4971(20)48341-7 [pii]
PST - ppublish
SO  - Blood. 2000 Nov 1;96(9):3126-32.