PMID- 11051717
OWN - NLM
STAT- MEDLINE
DCOM- 20001113
LR  - 20061115
IS  - 0379-4172 (Print)
IS  - 0379-4172 (Linking)
VI  - 27
IP  - 7
DP  - 2000
TI  - [A study of transgenic IFV cattle integrated with human serum albumin gene].
PG  - 573-9
AB  - The mammary gland expression vector (pcDNA 3.1-GCALBm) containing the full-length 
      sequence of human serum albumin (hALB) cDNA and intron 1 as well as the goat 
      beta-casien gene promoter and 5' up-stream regulatory sequence was constructed. 
      The vector was micro-injected into bovine IVF eggs. The embryos were in vitro 
      cultured to the late stage of morulae, and then few embryo cells were aspirated 
      for the implantation detection of target gene integration and SRY DNA sequence 
      using nested-PCR. Afterwards, ten integrated embryos were selected to transfer 
      into eight recipients and three were pregnant. The pregnant rate was 37.5%(3/8). 
      However, two were miscarried in mid-trimester but one was pregnant at term to 
      deliver a male transgenic cattle integrated with hALB mini-gene. The transgenic 
      efficiency was 12.5% (1/8).
FAU - Huang, S Z
AU  - Huang SZ
AD  - Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, China.
FAU - Huang, Y
AU  - Huang Y
FAU - Chen, M J
AU  - Chen MJ
FAU - Chen, W
AU  - Chen W
FAU - Huang, Z
AU  - Huang Z
FAU - Li, H
AU  - Li H
FAU - Li, J C
AU  - Li JC
FAU - Ren, Z R
AU  - Ren ZR
FAU - Zeng, Y T
AU  - Zeng YT
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yi Chuan Xue Bao
JT  - Yi chuan xue bao = Acta genetica Sinica
JID - 7900784
RN  - 0 (Serum Albumin)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Cattle/*genetics
MH  - Female
MH  - *Fertilization in Vitro
MH  - *Gene Transfer, Horizontal
MH  - Humans
MH  - Male
MH  - Organ Culture Techniques
MH  - Pregnancy
MH  - Serum Albumin/*genetics
EDAT- 2000/10/29 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/29 11:00
PHST- 2000/10/29 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/29 11:00 [entrez]
PST - ppublish
SO  - Yi Chuan Xue Bao. 2000;27(7):573-9.