PMID- 11052264
OWN - NLM
STAT- MEDLINE
DCOM- 20010215
LR  - 20191104
IS  - 0948-6143 (Print)
IS  - 0948-6143 (Linking)
VI  - 114
IP  - 2
DP  - 2000 Aug
TI  - Temporospatial expression of tissue inhibitors of matrix metalloproteinases-1, -2 
      and -3 during development, growth and aging of the mouse skeleton.
PG  - 157-65
AB  - Proteolytic degradation of collagen-rich extracellular matrices is a key feature 
      in the development, growth and aging of skeleton. Matrix metalloproteinases 
      (MMPs) are a family of enzymes capable of performing this function, whereas 
      tissue inhibitors of MMPs (TIMPs) are believed to play an important role in 
      regulating their activity. To better understand the roles of TIMP-1, -2 and -3, 
      we have studied their mRNA levels in several different mouse tissues with special 
      emphasis on the skeleton and the developing eye. A systematic analysis of TIMP-1, 
      -2 and -3 mRNA levels in mouse knee joints during growth and aging demonstrated 
      markedly different expression patterns for each TIMP. Immunohistochemical 
      analysis revealed several time-dependent changes in the distribution of TIMP-1 
      and -2 in articular and growth cartilages, synovial tissue and bone. The data 
      suggest that upon aging synovial tissue becomes the major source of synovial 
      fluid TIMPs. In articular cartilage these inhibitors were mainly found in the 
      deep layer and in subchondral bone. Compared with epiphyseal growth plate, the 
      amounts of TIMP-1 and -2 in articular cartilage were quite low. These findings 
      suggest that the capacity of articular cartilage chondrocytes to inhibit MMP 
      activities by local production of TIMPs is limited, which may be of consequence 
      during osteoarthritic cartilage degeneration.
FAU - Joronen, K
AU  - Joronen K
AD  - Department of Medical Biochemistry and Molecular Biology, University of Turku, 
      Finland.
FAU - Salminen, H
AU  - Salminen H
FAU - Glumoff, V
AU  - Glumoff V
FAU - Savontaus, M
AU  - Savontaus M
FAU - Vuorio, E
AU  - Vuorio E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Histochem Cell Biol
JT  - Histochemistry and cell biology
JID - 9506663
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-3)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
SB  - IM
MH  - Aging/*physiology
MH  - Animals
MH  - Bone and Bones/*chemistry
MH  - Cartilage, Articular/chemistry
MH  - Extremities/embryology
MH  - Eye/chemistry/embryology
MH  - Gene Expression
MH  - Growth Plate/chemistry
MH  - *Matrix Metalloproteinase Inhibitors
MH  - Mice
MH  - RNA, Messenger
MH  - Tissue Inhibitor of Metalloproteinase-1/analysis/*biosynthesis/genetics
MH  - Tissue Inhibitor of Metalloproteinase-2/analysis/*biosynthesis/genetics
MH  - Tissue Inhibitor of Metalloproteinase-3/analysis/*biosynthesis/genetics
EDAT- 2000/10/29 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/10/29 11:00
PHST- 2000/10/29 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/10/29 11:00 [entrez]
AID - 10.1007/s004180000177 [doi]
PST - ppublish
SO  - Histochem Cell Biol. 2000 Aug;114(2):157-65. doi: 10.1007/s004180000177.