PMID- 11053056
OWN - NLM
STAT- MEDLINE
DCOM- 20001207
LR  - 20211203
IS  - 1931-857X (Print)
IS  - 1522-1466 (Linking)
VI  - 279
IP  - 5
DP  - 2000 Nov
TI  - Spontaneous shift in transcriptional profile of explanted glomeruli via 
      activation of the MAP kinase family.
PG  - F954-9
AB  - To understand how isolation and explantation of glomeruli affect the function of 
      resident cells, the present study investigated the transcriptional profile of 
      explanted normal glomeruli. We found that ex vivo incubation of glomeruli 
      spontaneously expressed monocyte chemoattractant protein-1 (MCP-1) and 
      stromelysin, the genes regulated by activator protein-1 (AP-1). The expression 
      was suppressed by heparin and quercetin, the drugs with anti-AP-1 activities. The 
      gene expression was preceded by 1) induction of AP-1 components c-fos and c-jun 
      and 2) phosphorylation of extracellular signal-regulated kinase (ERK), p38 
      mitogen-activated protein (MAP) kinase, and c-Jun NH(2)-terminal kinase (JNK), 
      the upstream inducers/activators of AP-1. Suppression of ERK by PD098059 
      abrogated induction of c-fos and c-jun, and the p38 MAP kinase inhibitor SB203580 
      attenuated c-fos expression. Furthermore, treatment with either PD098059, 
      SB203580, or the JNK-AP-1 inhibitor curcumin diminished the expression of MCP-1 
      and stromelysin. The transcriptional profile of glomerular cells thus alters 
      dramatically after explantation of glomeruli. It is, at least in part, due to 
      activation of multiple MAP kinases that lead to induction of AP-1-dependent gene 
      expression.
FAU - Ishikawa, Y
AU  - Ishikawa Y
AD  - Renal Bioengineering Unit, Department of Medicine, University College Medical 
      School, University College London, The Rayne Institute, London WC1E 6JJ, United 
      Kingdom.
FAU - Konta, T
AU  - Konta T
FAU - Kitamura, M
AU  - Kitamura M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Am J Physiol Renal Physiol
JT  - American journal of physiology. Renal physiology
JID - 100901990
RN  - 0 (Chemokine CCL2)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factor AP-1)
RN  - 9005-49-6 (Heparin)
RN  - 9IKM0I5T1E (Quercetin)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - EC 1.14.13.39 (Nos3 protein, rat)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 1)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - EC 3.1.3.48 (Ptpro protein, rat)
RN  - EC 3.1.3.48 (Receptor-Like Protein Tyrosine Phosphatases, Class 3)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chemokine CCL2/biosynthesis/*genetics
MH  - Gene Expression/drug effects
MH  - Gene Expression Profiling
MH  - Heparin/pharmacology
MH  - JNK Mitogen-Activated Protein Kinases
MH  - Kidney Glomerulus/cytology/*metabolism
MH  - MAP Kinase Kinase 1
MH  - Male
MH  - Matrix Metalloproteinase 3/biosynthesis/*genetics
MH  - Membrane Proteins/biosynthesis/genetics
MH  - Mitogen-Activated Protein Kinase Kinases/antagonists & inhibitors
MH  - Mitogen-Activated Protein Kinases/antagonists & inhibitors/*metabolism
MH  - Nitric Oxide Synthase/biosynthesis/genetics
MH  - Nitric Oxide Synthase Type III
MH  - Phosphorylation/drug effects
MH  - Protein Serine-Threonine Kinases/antagonists & inhibitors
MH  - Protein Tyrosine Phosphatases/biosynthesis/genetics
MH  - Proto-Oncogene Proteins c-fos/biosynthesis/genetics
MH  - Proto-Oncogene Proteins c-jun/biosynthesis/genetics
MH  - Quercetin/pharmacology
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor-Like Protein Tyrosine Phosphatases, Class 3
MH  - Transcription Factor AP-1/antagonists & inhibitors/metabolism
MH  - Transcription, Genetic/physiology
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 2000/10/29 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/29 11:00
PHST- 2000/10/29 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/29 11:00 [entrez]
AID - 10.1152/ajprenal.2000.279.5.F954 [doi]
PST - ppublish
SO  - Am J Physiol Renal Physiol. 2000 Nov;279(5):F954-9. doi: 
      10.1152/ajprenal.2000.279.5.F954.