PMID- 11053307
OWN - NLM
STAT- MEDLINE
DCOM- 20001114
LR  - 20220309
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 41
IP  - 12
DP  - 2000 Nov
TI  - Colocalization of TrkB and brain-derived neurotrophic factor proteins in 
      green-red-sensitive cone outer segments.
PG  - 4014-21
AB  - PURPOSE: To examine the distribution of neurotrophins (NTs) and their catalytic 
      receptors in adult rat photoreceptors. METHODS: Immunocytochemistry and Western 
      blot analyses were performed using primary antibodies raised against NTs (nerve 
      growth factor [NGF], brain-derived neurotrophic factor [BDNF], NT-3, and NT-4/5) 
      and NT receptors (TrkA, TrkB, TrkC, and p75NTR). Double-labeling of retinal 
      sections with opsin-specific antibodies was performed to identify each 
      photoreceptor type. Competitive experiments using excess recombinant NT or Trk 
      receptors confirmed the binding specificity of each antibody. RESULTS: TrkB and 
      BDNF immunoreactivity was colocalized in cone outer segments. TrkB and BDNF were 
      detected in all green-red-sensitive cones, but not in blue-UV cones or rods, and 
      other NTs and NT receptors were not detected in any of the photoreceptor types. 
      CONCLUSIONS: The findings suggest a specific role for BDNF through its signaling 
      receptor TrkB in the function and maintenance of green-red cones, the predominant 
      cone type in the rat retina.
FAU - Di Polo, A
AU  - Di Polo A
AD  - Center for Research in Neuroscience, Montreal General Hospital Research Institute 
      and McGill University, Montreal, Quebec, Canada. dipoloa@patho.umontreal.ca
FAU - Cheng, L
AU  - Cheng L
FAU - Bray, G M
AU  - Bray GM
FAU - Aguayo, A J
AU  - Aguayo AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Rod Opsins)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Color Perception
MH  - Immunoenzyme Techniques
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/*metabolism
MH  - Retinal Cone Photoreceptor Cells/*metabolism
MH  - Rod Opsins/metabolism
EDAT- 2000/10/29 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/29 11:00
PHST- 2000/10/29 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/29 11:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2000 Nov;41(12):4014-21.