PMID- 11053652
OWN - NLM
STAT- MEDLINE
DCOM- 20001207
LR  - 20190720
IS  - 0304-3835 (Print)
IS  - 0304-3835 (Linking)
VI  - 160
IP  - 2
DP  - 2000 Nov 28
TI  - Induction of apoptosis in prostate cancer cell lines by a flavonoid, baicalin.
PG  - 219-28
AB  - The flavonoid baicalin (baicalein 7-D-beta-glucuronate), isolated from the dried 
      root of Scutellaria baicalensis Georgi (Huang Qin), is widely used in the 
      traditional Chinese herbal medicine for its anti-inflammatory, anti-pyretic and 
      anti-hypersensitivity effects. In the present study, we investigated the in vitro 
      effects of baicalin on the growth, viability, and induction of apoptosis in 
      several human prostate cancer cell lines, including DU145, PC-3, LNCaP and 
      CA-HPV-10. The cell viability after treating with baicalin for 2-4 days was 
      quantified by a colorimetric 3-(4, 
      5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-s ulfophenyl)- 
      2H-tetrazolium (MTS) assay. The results showed that baicalin could inhibit the 
      proliferation of prostate cancer cells. The responses to baicalin were different 
      among different cell lines, with DU145 cells being the most sensitive and LNCaP 
      cells the most resistant. Baicalin caused a 50% inhibition of DU145 cells at 
      concentrations of 150 microM or above. The inhibition of proliferation of 
      prostate cancer cells after a short period of exposure to baicalin was associated 
      with induction by apoptosis, as evidenced by the typical nuclear fragmentation 
      using Hoechst 33258 staining, terminal deoxynucleotidyl transferase-mediated dUTP 
      nick end labeling (TUNEL) labeling, DNA fragmentation, activation of caspase-3 
      and cleavage of poly-ADP-ribose polymerase (PARP). The results indicate that 
      baicalin has direct anti-tumor effects on human prostate cancer cells.
FAU - Chan, F L
AU  - Chan FL
AD  - Department of Anatomy, Basic Medical Sciences Building, The Chinese University of 
      Hong Kong, Shatin, New Territories, Hong Kong, People's Republic of China. 
      franky-chan@chuk.edu.hk
FAU - Choi, H L
AU  - Choi HL
FAU - Chen, Z Y
AU  - Chen ZY
FAU - Chan, P S
AU  - Chan PS
FAU - Huang, Y
AU  - Huang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (Androgens)
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavonoids)
RN  - 0 (Growth Inhibitors)
RN  - 347Q89U4M5 (baicalin)
SB  - IM
MH  - Androgens/physiology
MH  - Antineoplastic Agents, Phytogenic/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Cell Division/drug effects
MH  - DNA Fragmentation
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Flavonoids/*pharmacology
MH  - Growth Inhibitors/pharmacology
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Neoplasms, Hormone-Dependent/drug therapy/pathology
MH  - Prostatic Neoplasms/drug therapy/*pathology
MH  - Tumor Cells, Cultured
EDAT- 2000/10/29 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/29 11:00
PHST- 2000/10/29 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/29 11:00 [entrez]
AID - S0304383500005917 [pii]
AID - 10.1016/s0304-3835(00)00591-7 [doi]
PST - ppublish
SO  - Cancer Lett. 2000 Nov 28;160(2):219-28. doi: 10.1016/s0304-3835(00)00591-7.