PMID- 11054068
OWN - NLM
STAT- MEDLINE
DCOM- 20001109
LR  - 20231024
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 110
IP  - 4
DP  - 2000 Sep
TI  - Diagnostic utility of fluorescence in situ hybridization in mantle-cell lymphoma.
PG  - 856-62
AB  - Mantle-cell lymphoma (MCL) has a poorer prognosis than other small B-cell 
      lymphomas, thus a definitive diagnosis is essential. The t(11;14)(q13;q32) 
      associated with MCL juxtaposes portions of CCND1 (11q13) and IGH (14q32), 
      resulting in over-expression of cyclin D1. In this study, a highly sensitive 
      two-colour fluorescence in situ hybridization (FISH) method was developed to 
      detect t(11;14)(q13;q32) in nuclei isolated from paraffin-embedded tissue. 
      Twenty-three MCLs, 13 normal controls and nine small B-cell lymphomas other than 
      MCL were studied by FISH. Each MCL had been previously investigated to detect 
      genomic IGH-CCND1 fusion by polymerase chain reaction (PCR) using DNA extracted 
      from frozen tissue. The IGH-CCND1 fusion detection rate in the MCLs was 96% by 
      FISH compared with 35% by PCR. By FISH, one MCL and three small B-cell lymphomas 
      other than MCL harboured abnormalities involving only IGH. Less than 1% of cells 
      showed false-positive IGH-CCND1 fusion in normal specimens by FISH. Thus, this 
      highly sensitive FISH assay is very useful in confirming the diagnosis of MCL, 
      has wide applicability as it may be performed on both paraffin-embedded and fresh 
      tissue, and may also facilitate detection of translocations involving these loci 
      in tumours other than MCL.
FAU - Remstein, E D
AU  - Remstein ED
AD  - Division of Hematopathology, Mayo Clinic and Mayo Foundation, Rochester, MN 
      55905, USA.
FAU - Kurtin, P J
AU  - Kurtin PJ
FAU - Buno, I
AU  - Buno I
FAU - Bailey, R J
AU  - Bailey RJ
FAU - Proffitt, J
AU  - Proffitt J
FAU - Wyatt, W A
AU  - Wyatt WA
FAU - Hanson, C A
AU  - Hanson CA
FAU - Dewald, G W
AU  - Dewald GW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
SB  - IM
MH  - Case-Control Studies
MH  - *Chromosomes, Human, Pair 11
MH  - *Chromosomes, Human, Pair 14
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis
MH  - Lymph Nodes
MH  - Lymphoma, Mantle-Cell/*diagnosis
MH  - Palatine Tonsil
MH  - Pilot Projects
MH  - Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - *Translocation, Genetic
EDAT- 2000/10/29 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/29 11:00
PHST- 2000/10/29 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/29 11:00 [entrez]
AID - bjh2303 [pii]
AID - 10.1046/j.1365-2141.2000.02303.x [doi]
PST - ppublish
SO  - Br J Haematol. 2000 Sep;110(4):856-62. doi: 10.1046/j.1365-2141.2000.02303.x.