PMID- 11054073
OWN - NLM
STAT- MEDLINE
DCOM- 20001109
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 110
IP  - 4
DP  - 2000 Sep
TI  - Involvement of natural killer cells in patients with myelodysplastic syndrome 
      carrying monosomy 7 revealed by the application of fluorescence in situ 
      hybridization to cells collected by means of fluorescence-activated cell sorting.
PG  - 876-9
AB  - Monosomy 7 is the most frequent chromosome abnormality among patients with 
      secondary myelodysplastic syndrome (MDS). We used fluorescence in situ 
      hybridization (FISH) and fluorescence-activated cell sorting (FACS) in order to 
      clarify the lineage involvement. Four patients, three with de novo MDS and one 
      with secondary MDS, were enrolled in this study. Monosomy 7 was observed in 
      pluripotent stem cells (CD34(+)Thy-1(+)), and in B (CD34(+)CD19(+)) and T/natural 
      killer (NK) progenitors (CD34(+)CD7(+)). The number of abnormal cells of B 
      (CD19(+)) and T (CD3(+)) cells was below the cut-off value, but approximately 60% 
      of the NK cells (CD3-CD56(+)) contained monosomy 7 in three of the patients.
FAU - Miura, I
AU  - Miura I
AD  - Third Department of Internal Medicine, Akita University School of Medicine, 
      Akita, Japan. ikuo@med.akita-u.ac.jp
FAU - Kobayashi, Y
AU  - Kobayashi Y
FAU - Takahashi, N
AU  - Takahashi N
FAU - Saitoh, K
AU  - Saitoh K
FAU - Miura, A B
AU  - Miura AB
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Antigens, CD34)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Antigens, CD34
MH  - B-Lymphocytes
MH  - Chromosomes, Human, Pair 7
MH  - Female
MH  - Flow Cytometry
MH  - Hematopoietic Stem Cells/immunology
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Killer Cells, Natural/*immunology
MH  - Male
MH  - Middle Aged
MH  - Monosomy
MH  - Myelodysplastic Syndromes/genetics/*immunology
EDAT- 2000/10/29 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/29 11:00
PHST- 2000/10/29 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/29 11:00 [entrez]
AID - bjh2294 [pii]
AID - 10.1046/j.1365-2141.2000.02294.x [doi]
PST - ppublish
SO  - Br J Haematol. 2000 Sep;110(4):876-9. doi: 10.1046/j.1365-2141.2000.02294.x.