PMID- 11054801
OWN - NLM
STAT- MEDLINE
DCOM- 20001222
LR  - 20131121
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 62
IP  - 3
DP  - 2000 Nov 1
TI  - NT-3 weakly stimulates proliferation of adult rat O1(-)O4(+) 
      oligodendrocyte-lineage cells and increases oligodendrocyte myelination in vitro.
PG  - 329-35
AB  - The transplantation of fibroblasts, genetically modified to secrete 
      neurotrophin-3 (NT-3) and/or brain-derived neurotrophic factor (BDNF), into 
      spinal cord-injured rats increases the production of new oligodendrocytes and 
      myelination (McTigue et al. [1998] J. Neurosci. 18:5354-5365). This experiment 
      did not fully resolve whether the effect was exerted on oligodendrocyte 
      precursors or on oligodendrocytes, or whether there was stimulation of both 
      proliferation and differentiation of the oligodendrocyte lineage cells. To 
      clarify the effects of NT-3 and BDNF, adult rat spinal cord was dissociated to 
      produce cultures in which both oligodendrocyte precursors (O1(-)O4(+)) and 
      oligodendrocytes (O1(+)) were present. Thymidine labeling of cells was determined 
      in the presence and absence of added NT-3 and/or BDNF. In addition, the effect of 
      these neurotrophins on myelination was determined by treating purified adult 
      O1(+) oligodendrocyte/embryonic dorsal root ganglion (DRG) neuron cocultures with 
      neurotrophins, only during the myelination period. O1(+) oligodendrocyte 
      proliferation was not stimulated by NT-3 or BDNF; however, the proliferation of 
      O1(-)O4(+) cells was increased in NT-3-treated cultures to a labeling index (LI: 
      24 hr) of 15-20%. This effect was observed at 5 but not at 10 days in vitro. In 
      comparison, basic fibroblast growth factor (bFGF) induced the proliferation of 
      both O1(+) oligodendrocytes (LI approximately 60%) and O1(-)O4(+) cells (LI 
      approximately 75%). The amount of myelin formed in purified O1(+) 
      oligodendrocyte/DRG neuron cocultures was significantly increased in NT-3-treated 
      cultures compared to untreated cultures. These results indicate that NT-3 is 
      weakly but transiently mitogenic for adult-derived oligodendrocyte precursors and 
      support the suggestion that NT-3 promotes the maturation of O1(+) 
      oligodendrocytes into myelin-forming cells.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Yan, H
AU  - Yan H
AD  - The Miami Project to Cure Paralysis and Department of Neurological Surgery, 
      University of Miami School of Medicine, Miami, Florida 33136, USA.
FAU - Wood, P M
AU  - Wood PM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism/pharmacology
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Female
MH  - Ganglia, Spinal/cytology/drug effects/metabolism
MH  - Myelin Sheath/drug effects/*metabolism
MH  - Neurons/cytology
MH  - Neurotrophin 3/*metabolism/pharmacology
MH  - Oligodendroglia/cytology/drug effects/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord/cytology/drug effects/metabolism
MH  - Stem Cells/cytology/drug effects/metabolism
MH  - Thymidine/metabolism
EDAT- 2000/10/31 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/31 11:00
PHST- 2000/10/31 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/31 11:00 [entrez]
AID - 10.1002/1097-4547(20001101)62:3<329::AID-JNR2>3.0.CO;2-C [pii]
AID - 10.1002/1097-4547(20001101)62:3<329::AID-JNR2>3.0.CO;2-C [doi]
PST - ppublish
SO  - J Neurosci Res. 2000 Nov 1;62(3):329-35. doi: 
      10.1002/1097-4547(20001101)62:3<329::AID-JNR2>3.0.CO;2-C.