PMID- 11054804
OWN - NLM
STAT- MEDLINE
DCOM- 20001222
LR  - 20131121
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 62
IP  - 3
DP  - 2000 Nov 1
TI  - Brain-derived neurotrophic factor increases activity of NR2B-containing 
      N-methyl-D-aspartate receptors in excised patches from hippocampal neurons.
PG  - 357-62
AB  - Growth factors, including members of the neurotrophin gene family, play a central 
      role in the regulation of neuronal survival and differentiation during 
      development. In addition to these relatively long-term actions of neurotrophins, 
      recent studies have shown that these factors also rapidly modulate synaptic 
      transmission. Brain-derived neurotrophic factor (BDNF), in particular, regulates 
      both pre- and postsynaptic aspects of hippocampal synaptic transmission. The 
      postsynaptic effects include an increase in glutamate responsiveness, mediated by 
      the N-methyl-D-aspartate (NMDA) glutamate receptor subtype. It is not clear, 
      however, where BDNF-trkB signal transduction is initiated, because trkB receptors 
      are located in both pre- and postsynaptic membranes. In the present study, we 
      used excised membrane patches from cultured hippocampal neurons to determine 
      whether BDNF directly modulates postsynaptic NMDA receptor activity. The results 
      indicate that acute exposure to BDNF increases NMDA single channel open 
      probability via postsynaptic trkB receptors and that this effect is dependent on 
      the presence of the NR2B subunit of the NMDA receptor.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Levine, E S
AU  - Levine ES
AD  - Department of Pharmacology and Program in Neuroscience, University of Connecticut 
      Health Center, Farmington 06030-6125, USA. eslevine@neuron.uchc.edu
FAU - Kolb, J E
AU  - Kolb JE
LA  - eng
GR  - NS39167/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (NR2B NMDA receptor)
RN  - 0 (Piperidines)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 6OTE87SCCW (6-Cyano-7-nitroquinoxaline-2,3-dione)
RN  - 76726-92-6 (2-Amino-5-phosphonovalerate)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - R8OE3P6O5S (ifenprodil)
SB  - IM
MH  - 2-Amino-5-phosphonovalerate/pharmacology
MH  - 6-Cyano-7-nitroquinoxaline-2,3-dione/pharmacology
MH  - Adenosine Triphosphate/metabolism/pharmacology
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Membrane/metabolism
MH  - Cells, Cultured
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Guanosine Triphosphate/metabolism/pharmacology
MH  - Hippocampus/cytology/drug effects/*metabolism
MH  - N-Methylaspartate/pharmacology
MH  - Neurons/cytology/drug effects/*metabolism
MH  - Patch-Clamp Techniques
MH  - Piperidines/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/agonists/antagonists & inhibitors/*metabolism
MH  - Signal Transduction/drug effects
MH  - Synaptic Transmission/drug effects
EDAT- 2000/10/31 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/31 11:00
PHST- 2000/10/31 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/31 11:00 [entrez]
AID - 10.1002/1097-4547(20001101)62:3<357::AID-JNR5>3.0.CO;2-6 [pii]
AID - 10.1002/1097-4547(20001101)62:3<357::AID-JNR5>3.0.CO;2-6 [doi]
PST - ppublish
SO  - J Neurosci Res. 2000 Nov 1;62(3):357-62. doi: 
      10.1002/1097-4547(20001101)62:3<357::AID-JNR5>3.0.CO;2-6.