PMID- 11055557
OWN - NLM
STAT- MEDLINE
DCOM- 20010215
LR  - 20190818
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 211
IP  - 1-2
DP  - 2000 Aug
TI  - Evidence for the involvement of dietary lipids on the modulation of transforming 
      growth factor-beta1 in the platelets of male rats.
PG  - 145-52
AB  - Transforming growth factor beta1 (TGF-beta1), a multifunctional cytokine 
      participates in the proliferation and differentiation of various cell types. 
      Platelets are an important source of TGF-beta1 and are physiologically linked to 
      a variety of chronic illnesses including cancer, heart disease and inflammation. 
      It is well known that dietary lipids modulate platelet function. Whether dietary 
      lipids affect growth factor status of platelets is not known. This study 
      addresses the effect of dietary lipids on TGF-beta1 status of the platelets. Male 
      8 month-old Sprague Dawley rats were allocated to different diet groups. The high 
      fat diets ( 18% by weight) comprising of high fat beef tallow (HFB), high fat 
      corn oil (HFC), high fat fish oil (HFF) and high fat olive oil (HFO) and one low 
      fat diet containing low fat soybean oil (LFS) (5% by weight) were fed to the 
      experimental animals for 6 weeks. The TGF-beta1 status in the platelet lysate was 
      assessed by using the CCL-64 mink lung cell bioassay and by Western blot 
      analysis. Platelet lysates were evaluated for their ability to inhibit the growth 
      of the CCL-64 mink lung cells, unexpectedly platelet lysates stimulated growth. 
      The stimulatory effect of platelet lysate was in the order HFF > HFO > HFB > HFC 
      > LFS. Acidification of the lysates to activate the latent form of TGF-beta1 
      resulted in the loss of the growth stimulatory potential of the platelet lysates 
      in all the groups. Western blot analysis of the platelet lysates to detect the 
      level of TGF-beta1 protein demonstrated that HFB diet group had the highest level 
      of TGF-beta1 and the HFC diet group had the lowest level of TGF-beta1 and were 
      significantly different (p < 0.05) as compared to the other three diet groups. 
      These findings demonstrate that dietary lipids varying in their fatty acid 
      composition, profoundly affect the level of growth modulating constituents of the 
      platelets. Further studies are warranted to refine our understanding of the 
      effect of dietary constituents on the physiology of the platelets.
FAU - Adam, J M
AU  - Adam JM
AD  - Department of Foods and Nutrition, Department of Medicine, University of 
      Manitoba, Winnipeg, Canada.
FAU - Raju, J
AU  - Raju J
FAU - Khalil, N
AU  - Khalil N
FAU - Bird, R P
AU  - Bird RP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Cell Extracts)
RN  - 0 (Dietary Fats)
RN  - 0 (Lipids)
RN  - 0 (Tgfb1 protein, rat)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Animals
MH  - Biological Assay
MH  - Blood Platelets/drug effects/*metabolism
MH  - Blotting, Western
MH  - Body Weight
MH  - Cell Extracts/chemistry/pharmacology
MH  - Cell Line
MH  - Dietary Fats/*metabolism
MH  - *Lipid Metabolism
MH  - Lipids/*administration & dosage
MH  - Male
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transforming Growth Factor beta/*blood/pharmacology
MH  - Transforming Growth Factor beta1
EDAT- 2000/10/31 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/10/31 11:00
PHST- 2000/10/31 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/10/31 11:00 [entrez]
AID - 10.1023/a:1007104715824 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2000 Aug;211(1-2):145-52. doi: 10.1023/a:1007104715824.