PMID- 11055963
OWN - NLM
STAT- MEDLINE
DCOM- 20010104
LR  - 20240322
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 66
IP  - 11
DP  - 2000 Nov
TI  - Comparative 16S rRNA analysis of lake bacterioplankton reveals globally 
      distributed phylogenetic clusters including an abundant group of actinobacteria.
PG  - 5053-65
AB  - In a search for cosmopolitan phylogenetic clusters of freshwater bacteria, we 
      recovered a total of 190 full and partial 16S ribosomal DNA (rDNA) sequences from 
      three different lakes (Lake Gossenkollesee, Austria; Lake Fuchskuhle, Germany; 
      and Lake Baikal, Russia). The phylogenetic comparison with the currently 
      available rDNA data set showed that our sequences fall into 16 clusters, which 
      otherwise include bacterial rDNA sequences of primarily freshwater and soil, but 
      not marine, origin. Six of the clusters were affiliated with the alpha, four were 
      affiliated with the beta, and one was affiliated with the gamma subclass of the 
      Proteobacteria; four were affiliated with the 
      Cytophaga-Flavobacterium-Bacteroides group; and one was affiliated with the class 
      Actinobacteria (formerly known as the high-G+C gram-positive bacteria). The 
      latter cluster (hgcI) is monophyletic and so far includes only sequences directly 
      retrieved from aquatic environments. Fluorescence in situ hybridization (FISH) 
      with probes specific for the hgcI cluster showed abundances of up to 1.7 x 10(5) 
      cells ml(-1) in Lake Gossenkollesee, with strong seasonal fluctuations, and high 
      abundances in the two other lakes investigated. Cell size measurements revealed 
      that Actinobacteria in Lake Gossenkollesee can account for up to 63% of the 
      bacterioplankton biomass. A combination of phylogenetic analysis and FISH was 
      used to reveal 16 globally distributed sequence clusters and to confirm the broad 
      distribution, abundance, and high biomass of members of the class Actinobacteria 
      in freshwater ecosystems.
FAU - Glockner, F O
AU  - Glockner FO
AD  - Max-Planck-Institut fur Marine Mikrobiologie, Bremen, Germany. fog@mpi-bremen.de
FAU - Zaichikov, E
AU  - Zaichikov E
FAU - Belkova, N
AU  - Belkova N
FAU - Denissova, L
AU  - Denissova L
FAU - Pernthaler, J
AU  - Pernthaler J
FAU - Pernthaler, A
AU  - Pernthaler A
FAU - Amann, R
AU  - Amann R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Actinobacteria/*classification/genetics/isolation & purification
MH  - Animals
MH  - Bacteroides/classification/genetics/isolation & purification
MH  - Bacteroidetes/classification/genetics/isolation & purification
MH  - Cloning, Molecular
MH  - DNA, Bacterial/analysis/genetics
MH  - DNA, Ribosomal/analysis/genetics
MH  - Ecosystem
MH  - Fresh Water/*microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Plankton/microbiology
MH  - Proteobacteria/*classification/genetics/isolation & purification
MH  - RNA, Ribosomal, 16S/*genetics
MH  - Sequence Analysis, DNA
PMC - PMC92419
EDAT- 2000/10/31 11:00
MHDA- 2001/02/28 10:01
PMCR- 2000/11/01
CRDT- 2000/10/31 11:00
PHST- 2000/10/31 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/31 11:00 [entrez]
PHST- 2000/11/01 00:00 [pmc-release]
AID - 0913 [pii]
AID - 10.1128/AEM.66.11.5053-5065.2000 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2000 Nov;66(11):5053-65. doi: 
      10.1128/AEM.66.11.5053-5065.2000.