PMID- 11056009
OWN - NLM
STAT- MEDLINE
DCOM- 20001213
LR  - 20220409
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 185
IP  - 3
DP  - 2000 Dec
TI  - Downregulation of platelet-activating factor responsiveness during maturation of 
      human dendritic cells.
PG  - 394-400
AB  - Dendritic cells (DCs) are specialized antigen-presenting cells characterized by 
      their ability to migrate into target sites, process antigens, and activate naive 
      T-cells. Biological activities of platelet-activating factor (PAF) and the 
      cytokine macrophage inflammatory protein-3beta (MIP-3beta) as well as the mRNA 
      expression of their receptors were characterized in human DCs during 
      lipopolysaccharide (LPS)-promoted maturation. Platelet-activating factor induced 
      calcium transients, migration-associated actin polymerization response, and 
      chemotaxis in immature human dendritic cells differentiated in vitro from 
      monocytes with interleukin-4 and granulocyte macrophage colony stimulating 
      factor. In addition, RT-PCR experiments indicated mRNA expression of the PAF 
      receptor in these immature DCs. Cell studies and mRNA analyses further revealed 
      that immature DCs neither respond to MIP-3beta nor express its specific receptor, 
      CCR7. Induction of cell differentiation by LPS led to the loss of the mRNA 
      expression of the PAF receptor, accompanied by decreasing intracellular calcium 
      release, actin polymerization, and migration after stimulation with PAF. In 
      contrast, LPS treatment induced increasing responsiveness toward MIP-3beta and 
      mRNA expression of CCR7. Comparable data regarding mRNA expression of PAF 
      receptor and PAF responsiveness were also obtained with another maturation 
      protocol using TNFalpha instead of LPS. The direct comparison between the two 
      different protocols showed a slower decrease of PAF responsiveness induced by 
      TNFalpha than by LPS. These results show the loss of PAF responsiveness 
      associated with downregulation of PAF receptor mRNA expression during LPS- and 
      TNFalpha-induced maturation in human DCs. Therefore, these findings point to a 
      functional relevance of PAF in recruiting immature DCs, whereas MIP-3beta might 
      regulate the migration of DCs at a later stage of maturation.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Dichmann, S
AU  - Dichmann S
AD  - Department of Experimental and Clinical Dermatology, University of Freiburg, 
      Germany. dichmann@haut.ukl.uni-freiburg.de
FAU - Rheinen, H
AU  - Rheinen H
FAU - Panther, E
AU  - Panther E
FAU - Herouy, Y
AU  - Herouy Y
FAU - Czech, W
AU  - Czech W
FAU - Termeer, C
AU  - Termeer C
FAU - Simon, J C
AU  - Simon JC
FAU - Gebicke-Haerter, P J
AU  - Gebicke-Haerter PJ
FAU - Norgauer, J
AU  - Norgauer J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Platelet Activating Factor)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (platelet activating factor receptor)
SB  - IM
MH  - Antigen Presentation
MH  - Cell Differentiation/physiology
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/drug effects/*physiology
MH  - Humans
MH  - Platelet Activating Factor/*pharmacology/*physiology
MH  - Platelet Membrane Glycoproteins/*physiology
MH  - RNA, Messenger/analysis
MH  - *Receptors, Cell Surface
MH  - *Receptors, G-Protein-Coupled
MH  - Signal Transduction/drug effects/physiology
EDAT- 2000/10/31 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/10/31 11:00
PHST- 2000/10/31 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/10/31 11:00 [entrez]
AID - 10.1002/1097-4652(200012)185:3<394::AID-JCP9>3.0.CO;2-Z [pii]
AID - 10.1002/1097-4652(200012)185:3<394::AID-JCP9>3.0.CO;2-Z [doi]
PST - ppublish
SO  - J Cell Physiol. 2000 Dec;185(3):394-400. doi: 
      10.1002/1097-4652(200012)185:3<394::AID-JCP9>3.0.CO;2-Z.