PMID- 11056210
OWN - NLM
STAT- MEDLINE
DCOM- 20001228
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 882
IP  - 1-2
DP  - 2000 Nov 3
TI  - Different stimulatory opioid effects on intracellular Ca(2+) in SH-SY5Y cells.
PG  - 256-65
AB  - Present study revealed the stimulatory effects of delta opioid receptor on 
      intracellular Ca(2+) concentration ([Ca(2+)](i)) in SH-SY5Y cells. Fura-2 based 
      single cell fluorescence ratio (F345/F380) was used to monitor the fluctuation of 
      [Ca(2+)](i). Application of the selective delta-opioid receptor agonist alone, 
      [D-Pen(2,5)]-enkephalin (DPDPE), hardly had any effects on cells cultivated for 
      3-10 days. However, after the cells had been pre-stimulated with cholinoceptor 
      agonist, carbachol, variable calcium elevation was found in 59% of the cultures. 
      The response was naltridole-reversible and dose-dependent, and was abolished 
      completely by thapsigargin (TG) treatment but not by administration of CdCl(2) or 
      0-Ca(2+) bath solutions. DPDPE-mediated [Ca(2+)](i) elevation was abolished by 
      pertussis toxin (PTX) pretreatment but not cholera toxin (CTX), indicating 
      coupling via G proteins of G(i)/G(o) subfamily. In 17.5% of the responding cells, 
      biphase response was found which may be due to both the stimulatory and the 
      inhibitory effects of opioid. On the other hand, in acutely dissociated cells, 
      DPPDE alone induced [Ca(2+)](i) increase in 50% of the cultures. The probability 
      and the amplitude of the elevation were decreased considerably by application of 
      nifedipine or 0-Ca(2+) bath solution and was little affected by application of 
      TG. DPDPE activated [Ca(2+)](i) increase via a PTX-insensitive and CTX-sensitive 
      pathway suggesting coupling through G(s) subunit. All these indicated the opioid 
      modulated the intracellular Ca(2+) regulation system through different pathways. 
      SH-SY5Y cell line might be a suitable model for the investigation of the complex 
      mechanism which underlies opioid function.
FAU - Chen, L
AU  - Chen L
AD  - Institute of Biophysics and Biochemistry, Huazhong University of Science and 
      Technology, 430074, Wuhan, PR China. lychen@163.net
FAU - Zou, S
AU  - Zou S
FAU - Lou, X
AU  - Lou X
FAU - Kang, H G
AU  - Kang HG
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Analgesics, Opioid)
RN  - 0 (Calcium Channels)
RN  - 0 (Cholinergic Agonists)
RN  - 0 (Receptors, Opioid, delta)
RN  - 88373-73-3 (Enkephalin, D-Penicillamine (2,5)-)
RN  - 8Y164V895Y (Carbachol)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Analgesics, Opioid/*pharmacology
MH  - Calcium/*metabolism
MH  - Calcium Channels/*drug effects/physiology
MH  - Carbachol/pharmacology
MH  - Cholinergic Agonists/pharmacology
MH  - Enkephalin, D-Penicillamine (2,5)-/*pharmacology
MH  - Humans
MH  - Receptors, Opioid, delta/*drug effects/physiology
MH  - Tumor Cells, Cultured
EDAT- 2000/11/01 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/01 11:00
PHST- 2000/11/01 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/01 11:00 [entrez]
AID - S0006-8993(00)02904-8 [pii]
AID - 10.1016/s0006-8993(00)02904-8 [doi]
PST - ppublish
SO  - Brain Res. 2000 Nov 3;882(1-2):256-65. doi: 10.1016/s0006-8993(00)02904-8.