PMID- 11057100
OWN - NLM
STAT- MEDLINE
DCOM- 20001214
LR  - 20181130
IS  - 0867-7077 (Print)
IS  - 0867-7077 (Linking)
VI  - 67
IP  - 11-12
DP  - 1999
TI  - [Secretion of interleukin-2 (IL-2) and interferon (IFN gamma)in whole blood cell 
      culture stimulated with mitogens in patients with lung neoplasms].
PG  - 504-10
AB  - The aim of this study was to compare the ability of blood lymphocytes from lung 
      cancer patients to secrete interleukin-2 (IL-2) and interferon gamma (IFNg) upon 
      stimulation with mitogens with that of healthy donors. 42 patients with small 
      cell lung cancer (SCLC), 30 patients with non small cell lung cancer (NSCLC) and 
      30 healthy donors were studied. The test was done in lung cancer patients before 
      treatment. IL-2 and IFNg levels were measured with Elisa ready kits (Genzyme) in 
      the supernatants of whole blood culture after stimulation with Pokeweed (PWM) and 
      Phytohemagglutinin (PHA) mitogens. The results of the cytokine levels after 
      stimulation were not normally distributed and thus were transformed to logarthms 
      for statistical evaluation. The t-test for transformed results were used to asses 
      the difference between groups. The median level of IFNg in the supernatant of 
      whole blood cultures was significantly lower in lung cancer patients than in 
      healthy blood donors both after PWM as well as after PHA stimulation. When 
      patients with NSCLC and SCLC were regarded separately the lower level of IFNg in 
      comparison with healthy donors was found in the supernatant of the blood cultures 
      only after stimulation with PWM. The median level of IL-2 in the supernatant of 
      whole blood culture in lung cancer patients was lower than in healthy blood 
      donors only after PWM stimulation. The same was true for SCLC patients. In NSCLC 
      IL-2 levels were significantly lower after stimulation with PWM as well after PHA 
      stimulation. IN CONCLUSION: secretion of IL-2 and IFNg in whole blood culture 
      after mitogen stimulation in lung cancer patients is significantly lower than in 
      healthy donors. No significant differences between SCLC and NSCLC were found.
FAU - Szopinski, J
AU  - Szopinski J
AD  - III Kliniki Gruzlicy i Chorob Pluc, Immunologii Instytutu Gruzlicy i Chorob Pluc.
FAU - von Kleist, S
AU  - von Kleist S
FAU - Panorska, A
AU  - Panorska A
FAU - Roginska, E
AU  - Roginska E
FAU - Rogala, E
AU  - Rogala E
FAU - Rowinska-Zakrzewska, E
AU  - Rowinska-Zakrzewska E
LA  - pol
PT  - Clinical Trial
PT  - Controlled Clinical Trial
PT  - Journal Article
TT  - Wydzielanie interleukiny-2 (IL-2) i interferonu (IFN gamma) w hodowli z pelnej 
      krwi stymulowanej mitogenami u chorych na raka pluca.
PL  - Poland
TA  - Pneumonol Alergol Pol
JT  - Pneumonologia i alergologia polska
JID - 9302892
RN  - 0 (Interleukin-2)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (Pokeweed Mitogens)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Non-Small-Cell Lung/*metabolism
MH  - Carcinoma, Small Cell/*metabolism
MH  - Cells, Cultured
MH  - Female
MH  - Humans
MH  - Interferon-gamma/*metabolism
MH  - Interleukin-2/*metabolism
MH  - Lung Neoplasms/*metabolism
MH  - *Lymphocyte Activation/drug effects
MH  - Lymphocytes/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Phytohemagglutinins/pharmacology
MH  - Pokeweed Mitogens/pharmacology
EDAT- 2000/11/01 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/01 11:00
PHST- 2000/11/01 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/01 11:00 [entrez]
PST - ppublish
SO  - Pneumonol Alergol Pol. 1999;67(11-12):504-10.