PMID- 11057742
OWN - NLM
STAT- MEDLINE
DCOM- 20001113
LR  - 20190910
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 76
IP  - 10
DP  - 2000 Oct
TI  - Recombination between homologous chromosomes does not play a dominant role in the 
      formation of radiation-induced chromosomal aberrations.
PG  - 1343-8
AB  - PURPOSE: In mammalian cells, the relevance of homologous recombination in 
      radiation-induced double-strand break (DSB) repair is not yet well understood. In 
      the present work, the role of recombination between homologous chromosomes and 
      homology-directed repair of DSB were studied, using X-ray-induced chromosomal 
      aberrations as an end-point. MATERIALS AND METHODS: Human-hamster hybrid cells 
      containing one or two copies of human chromosome 8 were used. If recombination 
      between homologous chromosomes plays a dominant role in DSB repair, it is 
      expected that X-irradiation of cells with two copies of chromosome 8 would result 
      in a lower frequency of aberrations involving this chromosome compared with cells 
      with only one copy of chromosome 8. The aberrations involving human chromosome 8 
      were detected by fluorescence in situ hybridization (FISH). Furthermore, a 
      comparison between the hamster cell line XR-C1 (defective in non-homologous 
      repair), CHO-9 (the wild-type cells) and the cell line XR-C1#8 (in which the 
      defect of XR-C1 is complemented by human chromosome 8) was made to determine, 
      indirectly, the involvement of homology-directed recombination in DSB repair. 
      RESULTS: The observed frequencies of aberrations per human chromosome 8 were not 
      significantly different between cells containing one or two copies of this 
      chromosome. The frequency of chromatid-type aberrations was doubled in XR-C1 
      cells compared with CHO-9 and XR-C1#8 cells. CONCLUSIONS: In hamster cells, 
      recombination between homologous chromosomes appears not to have a major role in 
      the formation of radiation-induced chromosomal aberrations, while nonhomologous 
      repair seems to be important in both the G and G2 phases of the cell cycle.
FAU - Marcon, F
AU  - Marcon F
AD  - Laboratory of Comparative Toxicology and Ecotoxicology, Istituto Superiore di 
      Sanita, Rome, Italy. Marcon@iss.it
FAU - Boei, J J
AU  - Boei JJ
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - *Chromosome Aberrations
MH  - Cricetinae
MH  - DNA Damage
MH  - DNA Repair
MH  - G2 Phase
MH  - *Recombination, Genetic
MH  - X-Rays
EDAT- 2000/11/01 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/01 11:00
PHST- 2000/11/01 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/01 11:00 [entrez]
AID - 10.1080/09553000050151619 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 2000 Oct;76(10):1343-8. doi: 10.1080/09553000050151619.