PMID- 11058792
OWN - NLM
STAT- MEDLINE
DCOM- 20001222
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 294
IP  - 2
DP  - 2000 Nov 17
TI  - Phosphofructokinase, a glycolytic regulatory enzyme has a crucial role for 
      maintenance of synaptic activity in guinea pig hippocampal slices.
PG  - 81-4
AB  - To clarify the importance of glucose metabolism in maintaining neural activity, 
      we investigated the effects of lowering the concentration of glucose in perfusion 
      medium on synaptic activity (population spikes, PS) and on the level of high 
      energy phosphates in the region of dentate gyrus (DG), CA3 and CA1 area of 
      hippocampal slices of guinea pig. Further we determined the activity of 
      phosphofructokinase (PFK) and hexokinase (HK) of these areas. Lowering the 
      concentration of glucose from 10 to 5, 4, 3, 1 and 0 (mM) caused a reduction in 
      the PS amplitude. The PS in CA3 and CA1 region decayed and extinguished much 
      faster than that in DG area. The ATP and CrP levels in these three areas were 
      well preserved even after lowering the concentration of glucose to 3mM, and the 
      patterns of reduction showed no difference in these three areas. On the other 
      hand, there were significant differences in the activity of PFK in the DG, CA3 
      and CA1 areas, in contrast with HK which showed no significant differences. PFK 
      activity was highest in the DG area and followed by CA3 and CA1 regions. The 
      sensitivity in maintaining synaptic activity to lowered glucose concentrations 
      showed good parallels with the activity of PFK in these regions, indicating that 
      non-oxidative glycolytic metabolism regulated by PFK is crucial for the 
      maintenance of synaptic activity
FAU - Li, X
AU  - Li X
AD  - Department of Physiology, Kobe University School of Medicine, 7-5-1, Kusunoki-co, 
      Chuo-ku, 650-0017, Kobe, Japan.
FAU - Yokono, K
AU  - Yokono K
FAU - Okada, Y
AU  - Okada Y
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 020IUV4N33 (Phosphocreatine)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.11 (Phosphofructokinase-1)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Action Potentials/drug effects
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Dentate Gyrus/cytology/drug effects/enzymology
MH  - Dose-Response Relationship, Drug
MH  - Glucose/deficiency/metabolism/pharmacology
MH  - Glycolysis/drug effects/*physiology
MH  - Guinea Pigs
MH  - Hexokinase/metabolism
MH  - Hippocampus/cytology/drug effects/*enzymology
MH  - In Vitro Techniques
MH  - Phosphocreatine/metabolism
MH  - Phosphofructokinase-1/*metabolism
MH  - Synaptic Transmission/drug effects/*physiology
EDAT- 2000/11/04 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/04 11:00
PHST- 2000/11/04 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/04 11:00 [entrez]
AID - S0304-3940(00)01535-4 [pii]
AID - 10.1016/s0304-3940(00)01535-4 [doi]
PST - ppublish
SO  - Neurosci Lett. 2000 Nov 17;294(2):81-4. doi: 10.1016/s0304-3940(00)01535-4.