PMID- 11060013
OWN - NLM
STAT- MEDLINE
DCOM- 20001129
LR  - 20181223
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 19
IP  - 21
DP  - 2000 Nov 1
TI  - Structure of a covalently stabilized complex of a human alphabeta T-cell 
      receptor, influenza HA peptide and MHC class II molecule, HLA-DR1.
PG  - 5611-24
AB  - An alphabeta T-cell receptor (alphabetaTCR)/hemagglutinin (HA) peptide/human 
      leukocyte antigen (HLA)-DR1 complex was stabilized by flexibly linking the HA 
      peptide with the human HA1.7 alphabetaTCR, to increase the local concentration of 
      the interacting proteins once the peptide has been loaded onto the major 
      histocompatibility complex (MHC) molecule. The structure of the complex, 
      determined by X-ray crystallography, has a binding mode similar to that of the 
      human B7 alphabetaTCR on a pMHCI molecule. Twelve of the 15 MHC residues 
      contacted are at the same positions observed earlier in class I MHC/peptide/TCR 
      complexes. One contact, to an MHC loop outside the peptide-binding site, is 
      conserved and specific to pMHCII complexes. TCR gene usage in the response to 
      HA/HLA-DR appears to conserve charged interactions between three lysines of the 
      peptide and acidic residues on the TCR.
FAU - Hennecke, J
AU  - Hennecke J
AD  - Department of Molecular and Cellular Biology, Harvard University, Howard Hughes 
      Medical Institute, 7 Divinity Avenue, Cambridge, MA 02138, USA.
FAU - Carfi, A
AU  - Carfi A
FAU - Wiley, D C
AU  - Wiley DC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (HLA-DR1 Antigen)
RN  - 0 (Hemagglutinin Glycoproteins, Influenza Virus)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites/genetics
MH  - Crystallography, X-Ray
MH  - Drug Stability
MH  - HLA-DR1 Antigen/*chemistry/genetics
MH  - Hemagglutinin Glycoproteins, Influenza Virus/*chemistry/genetics
MH  - Humans
MH  - In Vitro Techniques
MH  - Macromolecular Substances
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Protein Conformation
MH  - Protein Engineering
MH  - Protein Folding
MH  - Receptors, Antigen, T-Cell, alpha-beta/*chemistry/genetics
MH  - Recombinant Proteins/chemistry/genetics
MH  - Sequence Homology, Amino Acid
MH  - Static Electricity
PMC - PMC305780
EDAT- 2000/11/04 11:00
MHDA- 2001/02/28 10:01
PMCR- 2001/11/01
CRDT- 2000/11/04 11:00
PHST- 2000/11/04 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/04 11:00 [entrez]
PHST- 2001/11/01 00:00 [pmc-release]
AID - cdd552 [pii]
AID - 10.1093/emboj/19.21.5611 [doi]
PST - ppublish
SO  - EMBO J. 2000 Nov 1;19(21):5611-24. doi: 10.1093/emboj/19.21.5611.