PMID- 11060137
OWN - NLM
STAT- MEDLINE
DCOM- 20010712
LR  - 20190513
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 528
IP  - Pt 3
DP  - 2000 Nov 1
TI  - Effect of prolonged, submaximal exercise and carbohydrate ingestion on monocyte 
      intracellular cytokine production in humans.
PG  - 647-55
AB  - The present study was undertaken to examine the effect of exercise and 
      carbohydrate (CHO) ingestion on intracellular monocyte cytokine production. 
      Subjects performed 2 h of cycling at 70 % peak pulmonary O2 uptake (VO2,peak) 
      while ingesting either an 8 % CHO beverage or a sweet placebo. Whole blood was 
      incubated with (stimulated) or without (spontaneous) lipopolysaccharide (LPS) and 
      surface stained for monocyte surface antigens. The cells were permeabilised, 
      stained for intracellular cytokines and analysed using flow cytometry. Exercise 
      had no effect on the number of monocytes spontaneously producing cytokines, but 
      the number of stimulated IL-1alpha-, TNF-alpha- and IL-6-positive monocytes were 
      elevated (P < 0.01) immediately post-exercise and 2 h post-exercise. These 
      stimulated cells produced less (P < 0.05) TNF-alpha immediately post-exercise, 
      and less (P < 0.05) TNF-alpha and IL-1alpha 2 h post-exercise. There was a small, 
      but significant increase (P < 0.05) in the plasma IL-6 concentration immediately 
      post-exercise. Exercise resulted in an elevation (P < 0.01) in the plasma 
      adrenaline concentration in the placebo trial, and ingestion of CHO attenuated 
      this increase. CHO ingestion had no effect on monocyte cytokine production, 
      plasma IL-6 or circulating leukocyte numbers. These data suggest that circulating 
      monocytes are not the origin of increased levels of plasma IL-6 during exercise: 
      prolonged cycling exercise increased the number of monocytes producing cytokines 
      upon stimulation, but these cells produced less cytokines post-exercise. In 
      addition, attenuation of plasma adrenaline levels had no effect on plasma IL-6 or 
      monocyte cytokine production.
FAU - Starkie, R L
AU  - Starkie RL
AD  - Exercise Physiology and Metabolism Laboratory, Department of Physiology, The 
      University of Melbourne, Parkville 3052, Victoria, Australia.
FAU - Angus, D J
AU  - Angus DJ
FAU - Rolland, J
AU  - Rolland J
FAU - Hargreaves, M
AU  - Hargreaves M
FAU - Febbraio, M A
AU  - Febbraio MA
LA  - eng
PT  - Clinical Trial
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Cytokines)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - YKH834O4BH (Epinephrine)
SB  - IM
MH  - Adult
MH  - Cytokines/*biosynthesis
MH  - Dietary Carbohydrates/*pharmacology
MH  - Epinephrine/blood
MH  - Exercise/*physiology
MH  - Humans
MH  - Interleukin-1/blood
MH  - Interleukin-6/blood
MH  - Intracellular Membranes/*metabolism
MH  - Male
MH  - Monocytes/drug effects/*metabolism
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC2270161
EDAT- 2000/11/04 00:00
MHDA- 2001/07/13 10:01
PMCR- 2001/11/01
CRDT- 2000/11/04 00:00
PHST- 2000/11/04 00:00 [pubmed]
PHST- 2001/07/13 10:01 [medline]
PHST- 2000/11/04 00:00 [entrez]
PHST- 2001/11/01 00:00 [pmc-release]
AID - PHY_1377 [pii]
AID - 10.1111/j.1469-7793.2000.t01-1-00647.x [doi]
PST - ppublish
SO  - J Physiol. 2000 Nov 1;528(Pt 3):647-55. doi: 
      10.1111/j.1469-7793.2000.t01-1-00647.x.