PMID- 11060453
OWN - NLM
STAT- MEDLINE
DCOM- 20001121
LR  - 20221207
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 90
IP  - 1-2
DP  - 2000
TI  - Spontaneous frequencies of aneuploid and diploid sperm in 10 normal Chinese men: 
      assessed by multicolor fluorescence in situ hybridization.
PG  - 79-83
AB  - Many studies have been published establishing the background frequencies of 
      disomic and diploid sperm in normal men by fluorescence in situ hybridization 
      (FISH) analysis, with highly significant variance among the reports. Besides 
      interdonor heterogeneity and differences in the experimental protocols used, the 
      question of inherent differences in chromosome malsegregation and meiotic arrest 
      among different geographic and ethnic groups of donors has been raised. In this 
      study, multicolor FISH analysis was carried out on semen samples from 10 
      nonsmoking, nondrinking Chinese men from the People's Republic of China. The 
      results were compared to FISH data on 10 nonsmoking, nondrinking Canadians under 
      the same experimental conditions, in the same laboratory. A total of 200,497 
      sperm was scored in the Chinese donors and compared to 202,320 sperm from 
      Canadian donors. Approximately 10,000 sperm per chromosome probe per donor were 
      analyzed. The mean hybridization efficiency was 99.99%. The frequencies of 
      X-bearing and Y-bearing sperm were not significantly different from the expected 
      50% for each individual and for the combined data from all donors (49.73% vs. 
      49.46%, P = 0.3946). The mean disomy frequencies (range) were 0.07% (0.02%-0.12%) 
      for chromosome 13, 0.18% (0.09%-0.19%) for chromosome 21, 0.05% (0. 01%-0.09%) 
      for 24,XX, 0.02% (0.01%-0.06%) for 24,YY, and 0.29% (0. 13%-0.49%) for 24,XY. The 
      mean diploidy frequency (range) was 0.38% (0.22%-0.73%) for 13-21 hybridizations 
      and 0.32% (0.07%-0.70%) for XY hybridizations. Highly significant interdonor 
      heterogeneity was found for diploidy (P = 0.0000) and for XY disomy (P = 0.0011), 
      but no age effect was observed in any category of disomic or diploid sperm. The 
      data reported here show no marked differences in disomy and diploidy frequencies 
      between the mainland Chinese and Canadian groups, if donor heterogeneity is taken 
      into account.
CI  - Copyright 2000 S. Karger AG, Basel.
FAU - Shi, Q
AU  - Shi Q
AD  - Department of Medical Genetics, Faculty of Medicine, University of Calgary, and 
      Genetics Department, Alberta Children's Hospital, Calgary, Alberta, Canada.
FAU - Martin, R H
AU  - Martin RH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aging/genetics
MH  - *Aneuploidy
MH  - Asian People/*genetics
MH  - Canada
MH  - China
MH  - *Diploidy
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Sex Chromosomes/genetics
MH  - Smoking
MH  - Spermatozoa/*metabolism
EDAT- 2000/11/04 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/04 11:00
PHST- 2000/11/04 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/04 11:00 [entrez]
AID - 15668 [pii]
AID - 10.1159/000015668 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 2000;90(1-2):79-83. doi: 10.1159/000015668.