PMID- 11062716
OWN - NLM
STAT- MEDLINE
DCOM- 20001211
LR  - 20131121
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 20
IP  - 5A
DP  - 2000 Sep-Oct
TI  - Binuclear cells induced by acridine orange in giant cell tumor of bone.
PG  - 3013-7
AB  - We have recently found the presence of many binuclear cells among isolated and 
      smeared cells in giant cell tumor of the bone (GCT). These binuclear cells are 
      possibly associated with the formation of multinuclear cells. Therefore, this 
      study was undertaken to clarify the mechanism of binucleation in GCT, using 
      primary culture cells exposed to acridine orange (AO) which is a fluorescent 
      vital staining dye for the cytoplasm and nucleus and which inhibits mitosis. The 
      cells were isolated from explants of fresh tumor materials obtained from two GCT 
      patients (GCT1 and GCT2). These cells were cultured in Dulbecco's modified Eagle 
      medium (DMEM) with 10% Fetal calf serum (FCS). After exposure to 0.5 microgram/ml 
      AO, for 0, 6, 24, 48, 96 and 144 hours the following parameters were 
      investigated: 1) cell growth rate (GR); 2) frequency of hyperdiploid cells (%HDC) 
      by DNA cytofluorometry; 3) mitotic index (MI); 4) BrdU labeling index (LI); 5) 
      frequency of binuclear cells (%BNC). Compared to the control cells which were 
      cultured in AO-free medium, the GR of both GCT cells exposed to AO was remarkably 
      inhibited. The MI was 0 from 24 to 144 hours. The %HDC was increased at 24 hours 
      and was maintained high until 144 hours. The LI was temporarily increased at 6 
      hours, but was decreased at 48 hours. The %BNC was gradually increased. AO 
      inhibited DNA synthesis and cell mitotic activity in cultured GCT cells and it 
      finally caused inhibition of cell growth. However, the frequencies of G2 arrest 
      cells and binuclear cells were increased. These results suggested that the 
      binuclear cells in GCT may be formed from G2 arrest cells by amitotic nuclear 
      division, but not by mitosis without cytoplasmic division, or by cell fusion.
FAU - Kusuzaki, K
AU  - Kusuzaki K
AD  - Department of Orthopaedic Surgery, Kyoto Prefectural University of Medicine, 
      Japan. kusu@kamogawa.pathol.kpu-m.ac.jp
FAU - Takeshita, H
AU  - Takeshita H
FAU - Murata, H
AU  - Murata H
FAU - Hashiguchi, S
AU  - Hashiguchi S
FAU - Nozaki, T
AU  - Nozaki T
FAU - Emoto, K
AU  - Emoto K
FAU - Ashihara, T
AU  - Ashihara T
FAU - Hirasawa, Y
AU  - Hirasawa Y
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Nucleic Acid Synthesis Inhibitors)
RN  - EC 3.1.3.2 (Acid Phosphatase)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acid Phosphatase/metabolism
MH  - Acridine Orange/metabolism/*pharmacology
MH  - Bone Neoplasms/*pathology
MH  - Cell Division/drug effects
MH  - *Cell Nucleus
MH  - Fluorescent Dyes/metabolism/*pharmacology
MH  - Giant Cell Tumor of Bone/*pathology
MH  - Humans
MH  - Mitosis/drug effects
MH  - Nucleic Acid Synthesis Inhibitors/metabolism/*pharmacology
MH  - Tumor Cells, Cultured
EDAT- 2000/11/04 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/04 11:00
PHST- 2000/11/04 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/04 11:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 2000 Sep-Oct;20(5A):3013-7.