PMID- 11063793
OWN - NLM
STAT- MEDLINE
DCOM- 20001204
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 121
IP  - 2
DP  - 2000 Sep
TI  - Establishment of a human malignant fibrous histiocytoma cell line, COMA. 
      Characterization By conventional cytogenetics, comparative genomic hybridization, 
      and multiplex fluorescence In situ hybridization.
PG  - 117-23
AB  - The human COMA cell line has been established from a storiform pleomorphic 
      malignant fibrous histiocytoma (MFH). As expected for this tumor type, a very 
      complex karyotype was observed after R-banding analysis. An extensive analysis by 
      24-color painting, comparative genomic hybridization (CGH), and fluorescence in 
      situ hybridization (FISH) was performed. Twelve complex marker chromosomes 
      recurrently observed were clearly identified; among them, three were 
      systematically present in all analyzed metaphases. Amplifications detected by CGH 
      were refined by FISH with probes specific for various candidate loci. A 
      significant aneuploidy and numerous micronuclei were observed, which could be 
      related to the anomalies of centriole numbers detected in a proportion of cells. 
      Such an analysis, performed on a series of MFH cell lines, would allow the 
      delineation of the genomic alterations specific for the oncogenesis or 
      progression of this complex tumor type or both.
FAU - Mairal, A
AU  - Mairal A
AD  - INSERM U509 Laboratoire de Pathologie Moleculaire des Cancers, Paris, France.
FAU - Chibon, F
AU  - Chibon F
FAU - Rousselet, A
AU  - Rousselet A
FAU - Couturier, J
AU  - Couturier J
FAU - Terrier, P
AU  - Terrier P
FAU - Aurias, A
AU  - Aurias A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Aged
MH  - Centrosome
MH  - Chromosomes, Artificial, Yeast
MH  - Female
MH  - Histiocytoma, Benign Fibrous/genetics/*pathology/ultrastructure
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Nucleic Acid Hybridization/*methods
MH  - Soft Tissue Neoplasms/genetics/*pathology/ultrastructure
MH  - Tumor Cells, Cultured
EDAT- 2000/11/07 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/07 11:00
PHST- 2000/11/07 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/07 11:00 [entrez]
AID - S0165-4608(00)00261-2 [pii]
AID - 10.1016/s0165-4608(00)00261-2 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2000 Sep;121(2):117-23. doi: 
      10.1016/s0165-4608(00)00261-2.